Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...
Patent
1995-06-07
1997-08-26
Gorgos, Kathryn L.
Chemistry: electrical and wave energy
Processes and products
Electrophoresis or electro-osmosis processes and electrolyte...
204456, 204466, 204470, 204606, 204616, G01N 2726, G01N 27447
Patent
active
056607024
ABSTRACT:
The present invention relates to an electrophoresis media comprising a gel and a compound capable of restricting the diffusion of water within the gel. Diffusion restricting compounds useful in the present invention include but are not limited to polyols, polymeric alcohols, polysaccharides, polyoxyethylene ethers, polyamines, polypeptides, gums, zwiterionic detergents and mixtures thereof. The present method relates to any electrophoresis media and includes but is not limited to polyacrylamide, agarose, starch, cellulose acetate and sepharose. A further embodiment of the present invention relates to a stacking gel wherein a diffusion restricting compound is used to prevent diffusion of water between the stack gel and resolving gel. The addition of a diffusion restricting compound to the stacking gel has the particular advantage of causing the stacking gel to become opaque, thereby facilitating the addition of samples to the gel. The present invention also relates to enclosing electrophoresis media within a vacuum sealed container. In yet a further embodiment of the present invention, the media enclosed in the vacuum sealed container further comprises a diffusion restricting compound.
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Glyko, Inc.
Gorgos Kathryn L.
Starsiak Jr. John S.
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