Ussing chamber

Chemical apparatus and process disinfecting – deodorizing – preser – Control element responsive to a sensed operating condition

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Details

73 38, 73 6447, 422 8202, 4352871, 4352885, B01L 300, G01N 1508

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active

057388261

ABSTRACT:
An apparatus which is in the nature of an Ussing chamber utilizing a discrete separation between the two chambers in which solutions used for testing tissue are located is described. The cartridge is adapted to receive small portions of the skin or tissue and is also adapted to expose each of the two surfaces of the tissue to the solution circulating in the chamber facing the tissue surface. The tissue holder is split into two halves and has holes in fluid communication with the fluid in the chamber facing the hole. The holder accommodates the tissue to be tested and when assembled and placed between the two chambers constitutes a liquid seal to maintain the liquids in each chamber separated. Ions can transfer from the tissue into each chamber depending upon the characteristics of the tissue and the nature of the solutions. The two halves of the chamber are slidably mounted on supports thus permitting the movement of the chambers away from one another further permitting the insertion or removal of the tissue holder.

REFERENCES:
patent: 4317726 (1982-03-01), Shepel
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Schoenwald et al., J. Pharm. Sci., 72(11), pp. 1266-1272 (1983).
Precision Instrument Design, "Single Unit Diffusion Chambers," pp. 1-4, Aug. 1992.
Ussing et.al.; Dec. 23, 1950; Active Transport of Sodium As the Source of Electric Current in the Short-Circuited Isolated Frog Skin; Acta Phys., Scandinav.; vol. 23; pp. 111-127.
Field, et.al.; May 1971; Ion Transport in Rabbit Ileal Musoca I. Na and Cl Fluxes and Short-Circuit Current; Am. Jrnl. Phsiol vol. 220; No. 5; pp. 1388-1396.
Powell, et. al.; Sep. 1972; Electrolyte Secretion By the Guinea Pig Ileum In Vitro; Am. Jrnl Physiol.; vol. 223; No. 3; pp. 531-537.
Rohde, et. al.; Oct. 1973; In Vitro Measurement of Ion Fluxes Across Biopsies of Human Jejunal Mucosa During Cholera; Jrnl Applied Physiol; vol. 35; No. 4; pp. 557-561.
Powell, et.al.; Jun. 1979; Aspirin-Stimulated Intestinal Electrolyte Transport in Rabbit Ileum In Vitro; Gastroenterology; vo. 76; No. 6; pp. 1429-1437.
Berschneider, et.al; Jan. 1988; Altered Intestinal Chloride Transport In Cystic Fibrosis; FASEB J.; 2:2625-2629.

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