Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1993-09-10
1996-02-13
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
435 691, 435 697, 4352402, 4352523, 4353201, 536 221, 536 231, 536 234, 536 235, C12N 902, C12N 500, C12P 2106, C07H 1900
Patent
active
054910846
ABSTRACT:
This invention provides a cell comprising a DNA molecule having a regulatory element from a gene, other than a gene encoding a green-fluorescent protein operatively linked to a DNA sequence encoding the green-fluorescent protein. This invention also provides a method for selecting cells expressing a protein of interest which comprises: a. introducing into the cells a DNAI molecule having DNA sequence encoding the protein of interest and DNAII molecule having DNA sequence encoding a green-fluorescent protein; b. culturing the introduced cells in conditions permitting expression of the green-fluorescent protein and the protein of interest; and c. selecting the cultured cells which express green-fluorescent protein, thereby selecting cells expressing the protein of interest. Finally, this invention provides various uses of a green-fluorescent protein.
REFERENCES:
Gould, S. J., and subrami, S., Anal. Biochem., 175:5 (1988) (Exhibit D).
Silhavy, T. J., and Beckwith, J. R., Microbiol. Rev., 49:398 (1985) (Exhibit E).
Stewart, G. S. A. B., and Williams, P., J. Gen. Microbiol., 138:1289 (1992) (Exhibit F).
Prasher et al. "Primary structure of the Dequorea victoria . . . " Gene 111 pp. 229-233 1992.
Glover "Expression of cloned genes in animal cells" Gene cloning pp. 179-213 1984.
Chalfie Martin
Prasher Douglas
Kim Hyosuk
The Trustees of Columbia University in the City of New York
Wax Robert A.
White John P.
Woods Hole Oceanographic Institution
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