Use of zwitterions to mobilize isoelectrically focused ampholyte

Details Use of zwitterions to mobilize isoelectrically focused ampholyte Use of zwitterions to mobilize isoelectrically focused ampholyte

1990-12-20

1992-05-05

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

2041801, C25B 100, B01D 5702

Patent

active

051104342

ABSTRACT:
Ampholytic solute zones which have been stabilized by isoelectrical focusing in a separation medium such as a capillary are mobilized by the introduction of a relatively large quantity of zwitterion to one of the electrode chambers. When a voltage is then applied between the electrode chambers as in the focusing stage, the zwitterion migrates into the separation medium in accordance with the isoelectric point of the zwitterion. The voltage is maintained to caused continuous migration of the zwitterion into the capillary, thereby forming an expanding zone which displaces the focused zones formed in the focusing stage and causes them to travel axially through the capillary toward a detection point or out of the capillary for purposes of recovery.

REFERENCES:
patent: 4725343 (1988-02-01), Hjerten et al.
patent: 4911808 (1990-03-01), Hjerten
S. Hjerten et al., J. Chromatog. 346:265-270 (1985).
S. Hjerten et al., J. Chromatog. 387:127-138 (1987).
S. Hjerten et al., J. Chromatog. 347:191-198 (1985).

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