Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Patent
1998-07-16
2000-01-25
Smith, Lynette R. F.
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
435419, 800279, 800288, 800294, 800298, A01H 500, C12N 514, C12N 1582
Patent
active
060181060
ABSTRACT:
Plants that accumulate the yeast polyadenylate binding protein (yPAB) display a range of abnormalities, including a characteristic chlorosis in leaves to a necrosis and pronounced inhibition of growth. The severity of these abnormalities reflects the levels of yeast PAB expression in the transgenic plants. In contrast, no obvious differences are seen in undifferentiated callus cultures that express the same range of yeast PAB. The expression of the yeast PAB1 gene in plants does not affect expression of the plant PAB gene family or alter poly(A) length in the total RNA population. It is proposed that the yeast PAB1 gene or its product interferes with as yet unidentified functions of PABs, which functions are manifest only in differentiated, developed plants. Surprisingly, transgenic plants expressing the yeast PAB1 gene are also observed to have a systemic acquired resistance (SAR) to bacterial, fungal and viral pathogens.
REFERENCES:
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Sachs AB, "A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding proteins: Domain structure and expression." Cell 45: 827-835, Jun. 1986.
Le, Hanh et al. "The Wheat Poly (A)-Binding Protein Functionally Complements pab 1 in Yeast, " Eur. J. Biochem., vol. 243, pp. 350-357 (1997).
Belostotsky, Dmitry A. et al. "Differential Organ-Specific Expression of Three Poly (A)-Binding-Protein Genes from Arabidopsis thaliana," Proc. Natl. Acad. Sci. USA, vol. 90, p. 6686-6690 (Jul. 1993).
Berger, Lloyd C. et al. "Translation of Poly (A)-Binding Protein mRNA is Regulated by Growth Conditions," Biochem. Cell Biol., vol. 70, pp. 770-778 (1992).
Anderson, James T. et al. "PUB1 is a Major Nuclear and Cytoplasmic Polyadenylated RNA-Binding Protein in Saccharomyces cerevisiae," Mol. Cell. Biol., vol. 13, No. 10, pp. 6102-6113 (Oct. 1993).
Hunt Arthur G.
Li Qing-Shun
Von Lanken Carol
Yang Jianjun
Nelson Amy J.
Smith Lynette R. F.
University of Kentucky Research Foundation
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