Use of Xenopus laevis oocytes a microincubators

Details Use of Xenopus laevis oocytes a microincubators Use of Xenopus laevis oocytes a microincubators

2006-11-14

2006-11-14

Paras, Jr., Peter (Department: 1632)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C435S347000, C435S373000

Reexamination Certificate

active

07135336

ABSTRACT:
The present invention concerns methods for de-differentiation and stabilize cells, such as progenitor cells, by introducing the cells into the cytoplasm of host oocytes, such asXenopus laevisoocytes. Advantageously, this method obviates the need for any nuclear transfer procedure, which is known to disrupt the chromosomal architecture of donor and recipient cells. The present invention also concerns host oocytes encapsulating cells that have been introduced into their cytoplasm. The present invention also concerns cells that have been removed from the host oocytes.

REFERENCES:
patent: 6011197 (2000-01-01), Strelchenko et al.
patent: 6215041 (2001-04-01), Stice et al.
patent: 6635802 (2003-10-01), Piedrahita et al.
patent: 6680199 (2004-01-01), Susko-Parrish et al.
patent: 2002/0142397 (2002-10-01), Collas et al.
patent: 2003/0044976 (2003-03-01), Dominko et al.
patent: 2004/0072288 (2004-04-01), Collas et al.
Byrne, J.A. et al. “Nuclei of adult mammalian somatic cells are directly reprogrammed to oct-4 stem cell gene expression by amphibian oocytes”Current Biology,2003, 13(14):1206-1213.
Dennis, C. “Take a cell, any cell . . .”Nature.2003, 426(6966):490-491.
Gonda, K. et al. “Reversible disassembly of somatic nucleoli by the germ cell proteins FRGY2a and FRGY2b”Nature Cell Biology,2003, 5(3):205-210.
Kikyo, N. et al. “Active remodeling of somatic nuclei in egg cytoplasm by the nucleosomal ATPase ISWI”Science,2000, 289:2360-2362.
Kirchhof, N. et al. “Expression pattern of Oct-4, in preimplantation embryos of different species”Biol. Reprod.,2000, 63:1698-1705.
Morrison, S. et al. “Telomerase activity in hematopoietic cells is associated with self-renewal potential”Immunity,1996, 5:207-216.
Pan, G. et al. “Stem cell pluripotency and transcription factor Oct4”Cell Research,2002, 12(5-6):321-329.
Pesce, M. and Scholer, H. “Oct-4: Control of totipotency and germline determination”Mol. Reprod. Dev.,2000, 55:452-457.
Schler, H. “Cell therapy: Receptor switches off pluripotency”Applied Genetics News,2001 22(5):article 8.
Schmieder, S. et al. “Characterization of the putative chloride channel xCIC-5 expressed inXenopus laevisoocytes and comparison with endogenous chloride currents”J. Physiol.,1998, 511(Pt 2):379-393.
Thomson, J. et al. “Embryonic stem cell lines derived from human blastocysis”Science,1998, 282(5391):1145-1147.
Thompson, J. et al. “Isolation of a primate embryonic stem cell line”Proc. Natl. Acad. Sci. USA,1995, 92:7844-7848.

Affiliated with

Also associated with

Rating

Use of Xenopus laevis oocytes a microincubators does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Use of Xenopus laevis oocytes a microincubators, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Use of Xenopus laevis oocytes a microincubators will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3631977