Use of uridine diphosphate glucose 4-epimerase

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S233000

Reexamination Certificate

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06890739

ABSTRACT:
The present invention relates to a new use of uridine diphosphate glucose 4-epimerase (also called uridine diphosphate galactose 4-epimerase), and a method of converting uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) to uridine diphosphate N-acetylgalactosamine (UDP-GalNAc) by using the said enzyme. The process for producing UDP-GalNAc by using the uridine diphosphate glucose 4-epimerase and the UDP-GalNAc supply system according to the present invention are practical and efficient, and greatly beneficial to the industries.

REFERENCES:
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Schroegel, O., et al. (1996) Acc, No. P55180.*
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Kenji Yamamoto et al., “Purification and some Properties of UDP-N-Acetyglucosamine 4-Epimerase fromBacillus subtilis”, Agric. Biol. Chem., vol. 49, No. 3, pp. 603-609, 1985.
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Luis Glaser, “The Biosynthesis of N-Acetylgalactosamine”, The Journal of Biological Chemistry, vol. 234, No. 11, pp. 2801-2805, Nov. 1959.
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B. B. Quimby et al., “Characterization of two Mutations Associated with Epimerase-Deficiency Galactosemia, by use of a Yeast Expression System for Human UDP-Galactose-4-Epimerase”, Am. J. Hum. Genet., vol. 61, pp. 590-598, 1997.
Databse Swissport, Oct. 1, 1996, Schrogel et al. UDP-glucose 4-epimerase, Database Accession No. P55180, XP002235638 *abstract*.

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