Use of uracil-DNA glycosylase in genetic analysis

Details Use of uracil-DNA glycosylase in genetic analysis Use of uracil-DNA glycosylase in genetic analysis

1997-10-29

2000-07-18

Zitomer, Stephanie

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 435183, 435196, 2041828, C12Q 168, C12P 1934, C12N 916, G01N 2726

Patent

active

060905530

ABSTRACT:
The present invention relates to a process for detecting the presence of at least one specific nucleic acid sequence in a sample containing a nucleic acid or a mixture of nucleic acids by amplifying the nucleic acid using polymerase chain reaction, cleaving the amplified products with uracil DNA glycosylase to obtain short DNA segments and detecting the DNA fragments by using reverse blot hybridization.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4800159 (1989-01-01), Mullis et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5035996 (1991-07-01), Hartley
patent: 5104792 (1992-04-01), Silver et al.
patent: 5599672 (1997-02-01), Liang et al.
Promotional Materials, BESS T-Scan.sup..TM. Mutation Detection & Localization Kit Flyer, Epicentre Technologies, received Sep. 29, 1997.
Kinard et al. Abstr Gen Meet. Am Soc Microbiol 92: 116 (abstract provided), 1992.
Hawkins et al., Nature Biotechnol. 15:803-804, Aug. 1997.

Affiliated with

Also associated with

Rating

Use of uracil-DNA glycosylase in genetic analysis does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Use of uracil-DNA glycosylase in genetic analysis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Use of uracil-DNA glycosylase in genetic analysis will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2034959