Use of untranslated region of osmotin gene to enhance...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C536S023100, C800S278000, C800S279000, C435S320100

Reexamination Certificate

active

08008466

ABSTRACT:
The present invention provides methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 5′ and 3′ untranslated regions of genes encoding osmotin proteins and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional procedures, resulting in enhanced expression of the selected coding region. In a preferred embodiment, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.

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Liu et al 2003 Nature Biotechnology 21:1222-1228, provided by Applicant.
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Dong Liu, Insect resistance conferred by 283-kDa Photorhabdus Luminescens protein TcdA inArabidopsis thaliana, Nature Biotechnology, Oct. 2003, pp. 1222-1228,vol. 21, No. 10, Dow AgroSciences LLC, vol. 21, No. 10, Indianapolis, Indiana.
Donald E. Nelson, Analysis of structure and transcriptional activation of an osmotin gene, Plant Molecular Biology 1992, pp. 577-588, vol. 19, 1992 Kluwer Academic Publisher, printed in Belgium.
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