Use of tRNA genes to stabilize the inheritance of unstable plasm

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4352523, 43525233, 4353201, C12P 2102, C12N 121, C12N 1570, C12N 1574

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active

056078420

ABSTRACT:
Expression systems are provided, where plasmids can be safely maintained in a prokaryotic host, by providing for a tRNA gene for an essential tRNA in a tRNA gene negative background. A non-selective medium can be employed to permit vigorous growth of the host and efficient expression of a protein of interest.

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