Use of translationally altered RNA to confer resistance to maize

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide confers pathogen or pest resistance

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

800301, 536 2372, 435468, A01H 500, C12N 504, C12N 1582

Patent

active

060404965

ABSTRACT:
The present invention provides methods and compositions for inhibiting virus infection in susceptible monocotyledonous plants. The methods and compositions involve the production of translationally altered forms of messenger RNA sequence derived from the inhibited virus. The invention further provides structural and organizational information for the genome of strain B of maize dwarf mosaic virus. Methods for inhibiting MDMV-B infection are taught. These methods include the generation of transformed plants containing chimeric genes capable of expressing either MDMV-B proteins or translationally altered forms of messenger RNA sequences produced by MDMV-B.

REFERENCES:
patent: 5034323 (1991-07-01), Jorgensen et al.
patent: 5283184 (1994-02-01), Jorgensen et al.
patent: 5428144 (1995-06-01), Blair et al.
Smith, Holly A., "Transgenic Plant Virus Resistance Mediated by Untranslatable Sense RNAs: Expression, Regulation, and Fate of Nonessential RNAs", The Plant Cell, 6: 1441-1453 (1994).
Bazan et al., "Viral cysteine proteases are homologous to the trypsin-like family of serine proteases: Structural and functional implications", Proc. Natl. Acad. Sci. USA, 85: 7872-7876 (1988).
Chasan, R., "Making Sense (Suppression) of Viral RNA-Mediated Resistance", The Plant Cell, 6: 1329-1331 (1994).
de Haan et al., "Characterization of RNA-Mediated Resistance to Tomato Spotted Wilt Virus in Transgenic Tobacco Plants", Bio/Technology, 10:1133-1337 (1992).
Domier, et al., "Potyviral Proteins Share Amino Acid Sequence Homology with Picorna-, Como-, and Caulimoviral Proteins", Virology, 158: 20-27 (1987).
Dougherty et al., "Expression and Function of Potyviral Gene Products", Ann. Rev. Phytopathol., 26: 123-143 (1988).
Dougherty et al., "RNA-Mediated Virus Resistance in Transgenic Plants" Exploitation of a Cellular Pathway Possibly Involved in RNA Degradation, Molecular Plant-Microbe Interactions 7(5):544-552 (1994).
Fang et al., "Genetic Engineering of Potyvirus Resistance Using Constructs Derived from the Zucchini Yellow Mosiac Virus Coat Protein Gene", Molecular Plant-Microbe Interactions, 6(3) 358-367 (1993).
Fitchen et al., "Genetically Engineered Protection Against Viruses in Transgenic Plants", Ann. Rev. Microbiol., 47:739-763 (1993).
Frenkel et al., "Unexpected sequence diversity in the amino-terminal ends of the coat proteins of strains of sugarcane mosiac virus", J. Gen. Virol., 72: 237-242 (1991).
Gorbalenya et al., "Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes", Nucleic Acids Research, 17(12): 4713-4730 (1989).
Gough et al., "Nucleotide Sequence of the Capsid and Nuclear Inclusion Protein Genes from the Johnson Grass Strain of Sugarcane Mosiac Virus RNA", J. Gen. Virol., 68: 297-304 (1987).
Huiet et al., "Nucleotide sequence and RNA hybridization analyses reveal an ambisense coding strategy for maize stripe virus RNA3", Virology, 182: 47-53 (1991).
Huiet et al., "Complete sequence of maize stripe virus RNA4 and mapping of its subgenomic RNAs", J. Gen. Virol., 73: 1603-1607 (1992).
Knoke et al., "Maize Dwarf Mosaic, Maize Chlorotic Dwarf, and Maize Streak", Plant Diseases of International Importance, I: 235-281, Prentice Hall (1992).
Lindbo et al., "Pathogen-Derived Resistance to a Potyvirus: Immune and Resistant Phenotypes in Transgenic Tobacco Expressing Altered Forms of a Potyvirus Coat Protein Nucleotide Sequence", Molecular Plant-Microbe Interactions, 5(2): 144-153 (1992).
Lindbo et al., "Untranslatable Transcripts of the Tobacco Etch Virus Coat Protein Gene Sequence Can Interfere with Tobacco Etch Virus Replication in Transgenic Plants and Protoplastss", Virology, 189: 725-733 (1992).
Lindbo et al., "Induction of a Highly Specifc Antiviral State in Transgenic Plants: Implications for Regulation of Gene Expression and Virus Resistance", The Plant Cell 5: 1749-1759 (1993).
Marzachi et al., "Cloning of the Maize Rough Dwarf Virus Genome: Molecular Confirmation of the Plant-Reovirus Classification Scheme and Identification of Two Large Nonoverlapping Coding Domains within a Single Genomic Segment", Virology, 180: 518-526 (1991).
Mirkov et al., "Engineering Sugarcane for Resistance to Sugarcane Mosaic Virus", Abstract P29-4, 1995 ASV Meeting, p. 178.
Morch et al., "A new `sense` RNA approach to block viral RNA replication in vitro", Nucleic Acids Research, 15(10) 4123-4130 (1987).
Mullineaux et al., "The nucleotide sequence of maize streak virus DNA", EMBO J., 3: 3063-3068 (1984).
Murry et al., "Transgenic Corn Plants Expressing MDMV Strain B Coat Protein are Resistant to Mixed Infections of Maize Dwarf Mosaic Virus and Maize Chlorotic Mottle Virus", Bio/Technology, 11: 1559-1564 (1993).
Nutter et al., "The complete nucleotide sequence of the maize chlorotic mottle virus genome", Nucleic Acids Research, 17: 3163-3177 (1989).
Oh et al., "Identification of Essential Residues in Potyvirus Proteinase HC-Pro by Site-Directed Mutagenesis", Virology, 173: 692-699 (1989).
Pang et al., "Different Mechanisms Protect Transgenic Tobacco Against Tomato Spotted Wilt and Impatiens Necrotic Spot Tospoviruses", Bio/Technology, 11: 819-824 (1993).
Poch et al., "Identification of four conserved motifs among the RNA-dependent polymerase encoding elements", EMBO, 8: 3867-3874 (1989).
Powell et al., "Protection against Tobacco Mosaic Virus Infection in Transgenic Plants Requires Accumulation of Coat Protein Rather than Coat Protein RNA Sequences", Virology 175: 124-130 (1990).
Riechmann et al., "Highlights and prospects of potyvirus molecular biology", Journal of General Virology, 73: 1-16 (1992).
Sohn et al., "Sequence analysis of the 3'-terminal half of RNA 1 of wheat spindle streak mosaic virus", Arch. Virol., 135: 279-292 (1994).
Van der Wilk et al., "Expression of the potato leafroll luteovirus coat protein gene in transgenic potato plants inhibits viral infection", Plant Mol. Biol., 17: 431-439 (1991).
Vardi et al., "Plants transformed with a cistron of a potato virus Y protease (Nia) are resistant to virus infection", Proc. Natl. Acad. Sci., 90: 7513-7517 (1993).
Vincent et al., "Nucleotide Sequence analysis and genomic organization of the NY-RPV isolate of barley yellow dwarf virus", J. Gen. Virol., 72: 2347-2355 (1991).
Wilson et al., "Plant Viruses: A Tool-Box for Genetic Engineering and Crop Protection", BioEssays, 10(6): 179-186 (1989).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Use of translationally altered RNA to confer resistance to maize does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Use of translationally altered RNA to confer resistance to maize, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Use of translationally altered RNA to confer resistance to maize will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-731871

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.