Use of the SP-2 monoclonal antibody for the clinical diagnostics

Details Use of the SP-2 monoclonal antibody for the clinical diagnostics Use of the SP-2 monoclonal antibody for the clinical diagnostics

1991-04-22

1994-03-29

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 71, 435 792, 435974, 530322, C12Q 170

Patent

active

052983919

ABSTRACT:
A method for in vitro immunoassaying an antigen from biological fluids or tissues. The antigen is present in individuals infected with the AIDS virus. The antigen is of cytoplasmic origin and is recognized by an SP-2 monoclonal antibody produced by a hybridoma cell line deposited at the Collection Nationale de Cultures de Microorganisms, Accession, No. I-1083. The method comprises the steps of taking a sample of biological fluid or tissue from a person; contacting the biological fluids or tissues with the antibody; removing an amount of the antibody not binding with the antigen; and determining the amount of the antibody bound with the antigen utilizing at least one revealing system selected from the group consisting of enzyme, radioisotope, and chemiluminescent labels.

REFERENCES:
patent: 4725669 (1988-02-01), Essex et al.
Iacobelli et al: "Detection of Antigens Recognized by a Novel MAb in Tissue and Serum from Patients with Breast Cancer" Cancer Res. 46, 3005-10 Jun. 1986.

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