Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification
Patent
1999-01-08
2000-11-07
Campell, Bruce R.
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
435 11, 435449, 435450, 435455, 800 8, 800 14, 800 21, C12N 1500, C12N 1502, C12N 1563, A01K 6700, A01K 67027
Patent
active
061435643
ABSTRACT:
The invention provides a method for obtaining a live offspring having maternal chromosomes derived from a live first polar body of an oocyte that has completed the first meiotic division. It has been discovered herein that chromosomes in the first polar body are able to participate in normal embryonic development if they are allowed to complete the second meiotic division within an enucleated mature oocyte, and are then allowed to mingle with chromosomes of a spermatozoon. The invention further provides a method for producing up to four embyros or live offspring having the chromosomes of a single oocyte, by using both the first polar body chromosomes, and the second polar body chromosomes to reconstitute recipient enucleated (fertilized) oocytes.
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Wakayama Teruhiko
Yanagimachi Ryuzo
Campell Bruce R.
Schnizer Richard
University of Hawai'i
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