Use of the oxygenase activity of acetolactate synthase for herbi

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

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435 25, 435189, 435190, 435817, C12Q 100, C12Q 126

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active

052061353

ABSTRACT:
A process for the detection of herbicides that inhibit acetolactate synthase (EC 4.1.3.18) by use of the enzyme, a suitable substrate (pyruvate, .alpha.-aceto-lactate, .alpha.-ketobutyrate, or .alpha.-aceto-.alpha.-hydroxybutyrate), and an oxygen-sensitive electrode is disclosed.

REFERENCES:
Schloss et al, Biochemistry, vol. 24, pp. 4952-4959, 1985.
Schloss et al, Nature, vol. 331, pp. 360-362, 1988.
Kobos et al, Trends in Biotechnology, vol. 7, pp. 101-105, 1989.
Sweet et al, Analytical Biochemistry, vol. 107, pp. 337-340, 1980.
Pelczar & Reid, Microbiology (McGraw-Hill, New York, 1972, pp. 159-162).
Enzyme Nomenclature 1978, Recommendations of the Nomenclature Committee of the International Union of Biochemistry on the Nomenclature and Classification of Enzymes, Academic Press.

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