Use of the GAL1 yeast promoter

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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536 27, 4353201, 435256, 435942, 435 695, 435 699, 435226, 435 6951, 435 694, 435 696, 4351723, 435 711, 935 6, 935 37, 935 60, 935 69, C12P 2102, C07H 1512, C12N 1512, C12N 1520, C12N 1518, C12N 964, C12N 118, C12N 1579, C12N 116

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051399369

ABSTRACT:
A DNA segment contains a GAL1 promoter of Saccharomyces cerevisae linked to a gene other than the galoctokinase gene, for directing the expression of the gene within a yeast cell.
A GAL1 promoter portion of Saccharomyces cerevisae is linked to a foreign DNA segment for use in expressing a desired protein. Yeast cells containing the GAL1 promoter linked to a foreign DNA segment are grown in a medium containing glucose, wherein the yeast cells metabolize the glucose and are permitted to express a polypeptide when galactose is present in the medium.

REFERENCES:
patent: 4443539 (1984-04-01), Fraser et al.
patent: 4468464 (1984-08-01), Cohen et al.
St. John et al. 1981. J. Mol. Biol. 152, 317-334.
Botstein, et al. 1982. in: The Molecular Biology of the Yeast Saccharomyces. Metabolism and Gene Expression. (Strathern etal. eds.) Cold Spring Harbor Laboratory. Cold Spring Harbor, N.Y. pp. 607-636.
Tuite et al, EMBO Journal vol. 1 No. 5 pp. 603-608 (1982).
Hitzeman et al, Nature vol. 293 pp. 717 to 722 Oct. 29, 1981.

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