Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Process involving micro-organisms of different genera in the...
Reexamination Certificate
2009-06-24
2011-10-04
Lilling, Herbert J (Department: 1657)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Process involving micro-organisms of different genera in the...
C435S135000, C435S170000
Reexamination Certificate
active
08030021
ABSTRACT:
A method for inexpensive and efficient PHA biosynthesis includes operating a sequencing bioreactor in alternating phases of nutrient deprivation and carbon feedstock deprivation to select for robust PHA-producing microbes. Preferably, the bioreactor is operated in a non-sterile manner with mixed cultures of methanotrophs. The method also preferably uses periodic biomass-wasting (PHA harvesting) at the end of the carbon feed phase, gradually lengthening the time period of carbon deprivation phase to create a penalty for rapid PHA degradation and incentive for PHA accumulation. Also, bacterial enrichment cultures may be introduced periodically. The PHA-accumulating bacteria are preferably grown on common anaerobic degradation products, specifically volatile fatty acids, such as acetate and propionate, and methane gas. The PHA has useful applications in bioplastics and other products.
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Criddle Craig S.
Pieja Allison J.
Lilling Herbert J
Lumen Patent Firm
The Board of Trustees of the Leland Standford Junior University
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