Use of Saccharomyces cerevisiae erg4 mutants for...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving glucose or galactose

Reexamination Certificate

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C435S029000, C435S254210, C435S320100, C530S350000, C536S023500, C536S023740

Reexamination Certificate

active

07615360

ABSTRACT:
The invention relates to yeast strains in which a human GLUT4 transport or a human GLUT1 transporter can be functionally expressed and to particular GLUT4 transport proteins which can be functionally expressed particularly readily in yeast strains.

REFERENCES:
patent: 6346374 (2002-02-01), Tartaglia
patent: 2004/0101848 (2004-05-01), Ward
patent: 2005/0147987 (2005-07-01), Venter et al.
patent: WO 00/75188 (2000-12-01), None
patent: WO 02/064784 (2002-08-01), None
Burns Nancy et al., Large-Scale Analysis Of Gene Expression, Protein Localization, And Gene Disruption inSaccharomyces cerevisiae, Genes & Development, (1994), vol. 8, pp. 1087-1105.
Buse, John B. et al., Human GLUT4/Muscle-Fat Glucose-Transporter Gene, Diabetes, (1992), vol. 41, pp. 1436-1445.
Fukumoto et al., Cloning and Characterization of the Major Insulin-responsive Glucose Transporter Expressed in Human Skeletal Muscle and Other Insulin-responsive Tissues, Journal of Bio. Chem., vol. 264, No. 15, May 15, 1989, pp. 7776-7779.
Wieczorke Roman et al., Concurrent Knock-Out Of At Least 20 Transporter Genes Is Required To Block Uptake Of Hexoses inSaccharomyces cerevisiae, FEBS Letter, (1999), vol. 464, pp. 123-128.

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