Use of RNA polymerase to improve nucleic acid amplification proc

Details Use of RNA polymerase to improve nucleic acid amplification proc Use of RNA polymerase to improve nucleic acid amplification proc

1997-01-28

2000-02-15

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435 9121, C12Q 168, C12P 1934

Patent

active

060251344

ABSTRACT:
This invention relates to the use of a eukaryotic or prokaryotic DNA-directed RNA polymerase of a class that synthesizes cellular RNA, in a process for the amplification of a specific nucleic acid sequence or of its complement. It also relates to a new process for amplifying a specific nucleic acid sequence. The process includes one or more reactions which may take place in a single reaction vessel. In one instance, in a first reaction, the process includes providing a first RNA polymerase which uses a DNA first template to synthesize an RNA first template, and, in a second reaction, providing the RNA first template and a number of other reagents such that the reagents use the RNA first template to synthesize a DNA second template and an RNA second template. Thereafter a cycle ensues in which the reagents use the RNA second template to synthesize a DNA third template and multiple copies of the RNA second template. The RNA second template is the specific nucleic acid sequence or its complement. This invention includes a kit containing the reagents of this invention.

REFERENCES:
patent: 5130238 (1992-07-01), Malek et al.
patent: 5409818 (1995-04-01), Davey et al.
Boom, R. et al. "Rapid and Simple Method for Purification of Nucleic Acids", 1990 J Clin Micro. 28:495-503.
Chamberlin, M.J. 1976. "RNA Polymerase" in Losick, R. and Chamberlin, M. (eds) in Cold Spring Harbor Laboratory, p. 17-67.
Ovchinnikov, Y.A. et al., 1977. "The Primary Structure of .alpha.-Subunit of DNA-Dependent RNA Polymerase from Escherichia coli", FEBS Lett. 76:108-111.
Sambrook, J. et al., 1989. "Molecular Cloning: a laboratory manual", 2nd ed. Cold Spring Harbor, NY: CSH Laboratory Press, p. 9.16-9.19 and p. 11.31-33.
Sooknanan, R. et al., 1993. "Detection and direct sequence identification of a BCR-ABL mRNA in Ph.sup.+ chronic myeloid leukemia" Experimental Hematology 21:1719-1724.
Thrash et al., J. Biolog. Chem. 252(16), 5615-5618 (1977).
McClure et al., J. Biol. Chem. 253(24), 8949-8956 (1978).
Guatelli et al., PNAS 87, 1874-1878 (1990).
Kwoh et al., PNAS 86, 1173-1177 (1989).
Kievits et al., J. Virol. Methods 35, 273-286 (1991).

Affiliated with

Also associated with

Rating

Use of RNA polymerase to improve nucleic acid amplification proc does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Use of RNA polymerase to improve nucleic acid amplification proc, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Use of RNA polymerase to improve nucleic acid amplification proc will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1904735