Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-06-07
1997-07-29
Fleisher, Mindy
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 9141, 435 9151, 536 253, 536 254, C12P 1934, C12N 1566, C07H 2100, C07H 2102
Patent
active
056521260
ABSTRACT:
The present invention relates to methods of cleaving phosphorothioate oligonucleotides. In particular, it relates to the use of certain restriction endonucleases to cleave phosphorothioate oligonucleotides which contain restriction endonuclease recognition sequences. These restriction sequences can be used to facilitate the cleavage of relatively cleavage resistant phosphorothioate oligonucleotides thus facilitating their separation and purification after synthesis.
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Dattagupta Nanibhushan
Kacian Daniel Louis
Lackey David Bruce
Fisher Carlos A.
Fleisher Mindy
Gen-Probe Incorporated
Weiss Bonnie D.
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