Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-03-22
2005-03-22
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200
Reexamination Certificate
active
06869760
ABSTRACT:
This invention provides a method for detecting cancer cells in a sample comprising detection of the expression of a Prostate Tumor Inducing Gene in the sample, wherein a positive detection of the expression indicates the presence of cancer cells in the sample. In an embodiment, the expression is performed by measuring the level of PTI mRNA. The mRNA is measured by reverse transcription-polymerase chain reaction using at least a pair of appropriate primers. It is well known in the art that once the sequence of the Prostate Tumor Inducing Gene is determined, appropriate pair of primers may be selected. This invention also provides the above method, wherein the expression is performed by measuring the level of PTI protein. The level of PTI protein is measured by steps of: a) contacting the sample with antibody capable of specifically recognizing PTI-1 protein under conditions permitting formation of complexes between the PTI-1 protein and the antibody; and b) measuring the complex formed, thereby measuring the level of PTI-1 protein expressed in the cancer cells.
REFERENCES:
patent: 5162504 (1992-11-01), Horoszewicz
patent: 5227471 (1993-07-01), Wright
patent: 5314996 (1994-05-01), Wright
patent: 5851764 (1998-12-01), Fisher et al.
patent: 6200765 (2001-03-01), Murphy et al.
patent: 9621671 (1996-07-01), None
Su Z et al., 1996, “Surface-epitope masking and expression cloning identifies the human prostate carcinoma tumor antigen gene PCTA-1 a member of the galectin gene family”Proc. Natl. Acad. Sci. USA 93: 7252-7257.
Lin J et al., 1996, “The 5'-UTR of prostate tumor inducing gene-1, PTI-1, is transcriptionally activated during oncogenic transformation” Proceedings Am. Assoc. Cancer Res. Ann. 37:146 (abstract 1008).
Veronese ML et al., 1996, “The t(6;16)(p21;q22) chromosome translocation in the LNCaP prostat carcinoma cell line results in tpc/hpr fusion gene”Cancer Res. 56:728-732.
Cama C et al., 1995, “Molecular staging of prostate cancer II. A comparison of the application of an enhanced reverse transcriptase polymerase chain reaction assay for prostate specific antigen versus prostate specific membrane antigen”J. Urol. 153:1373-1378.
Chastel C, 1995, “Links and interactions between mycoplasmas and viruses: past confusions and present realities”Arch. Virol. 140:811-826.
Jiang H et al., 1995, “The melanoma differentiation-associated gene mda-6, which encodes the cyclin-dependent kinase inhibitor p21, is differentially expressed during growth, differentiation and progression in human melanoma cells”Oncogene 10:1855-1864.
Shen R et al., 1995, “Identification of the human prostatic carcinoma oncogene PTI-1 by rapid expression cloning and differential RNA display”Proc. Natl. Acad. Sci. U.S.A. 92:6778-6782.
Tsai S et al., 1995, “Mycoplasmas and oncogenesis: persistent infection and multistage malignant transformation”Proc. Natl. Acad. Sci. U.S.A. 92:10197-10201.
Israeli RS et al., 1994, “Sensitive nested reverse transcription polymerase chain reaction detection of circulating prostatic tumor cells: comparison of prostate-specific membrane antigen and prostate-specific antigen-based assays”Cancer Res. 54:6306-6310.
Katz AE et al., 1994, “Molecular staging of prostate cancer with the use of an enhanced reverse transcriptase-PCR assay”Urol. 43:765-775.
Nakhla AM et al., 1994, “Characterization of ALVA-4 cells, a new human prostatic cancer cell line”Steroids 59(10):586-589.
Crozat A et al., 1993, “Fusion of CHOP to a novel RNA-binding protein in human myxoid liposarcoma”Nature 363:640-644.
Epstein JI et al., 1993, “Correlation of pathologic findings with progression after radical retropubi prostatectomy”Cancer 71:3582-3593.
Jiang H et al., 1993, “Use of a sensitive and efficient subtraction hybridization protocol for the identification of genes differentially regulated during the induction of differentiation in human melanoma cells”Mol. Cell. Different. 1(3):285-299.
Lu-Yao GL et al., 1993, “An assessment of radical prostatectomy. Time trends, geographic variation and outcomes. The Prostate Patient Outcomes Research Team”JAMA 269:2633-2636.
Liang R et al., 1992, “Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction”Science(Washington DC)257:967-971.
Su Z et al., 1992, “Transfer of a dominant-acting tumor-inducing oncogene from human prostatic carcinoma cells to cloned rat embryo fibroblast cells by DNA-transfection”Anticancer Res. 12:297-304.
Noller HF, 1991, “Ribosomal RNA and translation”Annu. Rev. Biochem. 60:191-227.
Mukamel E et al., 1987. “Pitfalls in preoperative staging in prostate cancer”Urol. 30:318-323.
Salo JO et al., 1987, “Computerized tomography and transrectal ultrasound in the assessment of local extension of prostatic cancer before radical retropubic prostatectomy”J. Urol. 137:435-438.
Shima ZA et al., 1986, “Gene encoding the a chain of the T-cell receptor is moved immediately downstream of c-myc in a chromosomal 8;14 translocation in a cell line from a human T-cell leukemia”Proc.Natl. Acad. Sci. U.S.A. 83:3439-3443.
Watt KWK et al., 1986, “Human prostate-specific antigen: structural and functional similarity wit serine proteases”Proc. Natl. Acad. Sci. U.S.A. 83:3166-3170.
Wang MC et al., 1979, “Purification of a human prostate specific antigen”Invest. Urol. 17:159-163.
Baker & Botts L.L.P.
Horlick Kenneth R.
The Trustees of Columbia University in the City of New York
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