Use of primers containing nucleotides having altered base pairin

Details Use of primers containing nucleotides having altered base pairin Use of primers containing nucleotides having altered base pairin

1996-11-25

1999-02-09

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435 9152, C12Q 168, C12P 1934

Patent

active

058692517

ABSTRACT:
The present invention provides improved methods for amplifying a nucleic acid molecule. More specifically, the invention involves replacing at least one nucleotide of an oligonucleotide with a nucleotide having altered base pairing characteristics, so as to more equalize the efficiency with which that oligonucleotide and a second oligonucleotide hybridize to a target molecule and then amplifying the target molecule using, for example, the polymerase chain reaction. Improved amplification results from the improvement in relative hybridization efficiencies.

REFERENCES:
patent: 4683194 (1987-07-01), Saiki et al.
patent: 4683202 (1987-07-01), Mullis
Butzer et al., Genomics 9, 481-487 (1991).
Meinkoth et al., Analyt. Biochem. 138, 267-284 (1984).
Batzer et al., Nucl. Acids Res. 19(18), 5081 (1991).
Ehlen et al., Biochem. Biophys. Res. Comm. 160(2), 441-447 (1989).
Loh et al., Science 243, 217-220 (1989).
Martin et al., Nucl. Acids Res. 13(24), 8927-8938 (1985).
Ohtsuka et al., J. Biol. Chem. 260(5), 2605-2608 (1985).
Varshney et al., Nucl. Acids Res. 16(9), 4162 (1988).
Barany, Proc. Natl. Acad. Sci USA 88, 189-193 (1991).
Knoth, K. et al., Nucleic Acids Research 16:10932 (1988).
Frohman, M.A. et al., Proc. Natl. Acad. Sci. (U.S.A.) 85:8998-9002 (1988).
Frohman, M.A., PCR Protocols, A Guide to Methods and Applications pp. 28-38 (Academic Press, NY, 1990).
Loh, E.Y., et al., Science 243:217 (1989).
Ohara, O. et al., Proc. Natl. Acad. Sci. (U.S.A.) 86:5673 (1989).
Fordham-Skelton, A.P. et al., Molec. Gen. Genet. 221:134-138 (1990).
Patil, R.V., Nucl. Acids Res. 18:3080 (1990).
Barker, R., Organic Chemistry of Biological Molecules Prentice Hall, NJ (1971), pp. 331-336.
Mullis, K. et al., Cold Spring Harbor Symp. Quant. Biol. 51:263-273 (1986).
Saiki, R.K. et al., Bio/Technology 3:1008-1012 (1985).
Shymala, V. et al., Gene 84:1-8 (1989).
Tannich, E. et al., Anal. Biochem. 188:255-258 (1989).

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