Use of polyoxyethylene ethers to improve the performance of immu

Details Use of polyoxyethylene ethers to improve the performance of immu Use of polyoxyethylene ethers to improve the performance of immu

1987-03-30

1989-03-07

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 28, 435188, 436511, 436825, 436175, G01N 33571, G01N 100, C12Q 128, C12N 996

Patent

active

048106305

ABSTRACT:
Signal to noise ratio of enzyme immunoassays employing peroxidase conjugates can be improved by including polyoxyethylene ether detergent in the assay buffer. Such detergents reduce interference due to blood and also improve sensitivity of assays of blood-free samples.

REFERENCES:
patent: 4454232 (1984-06-01), Breglio et al.
patent: 4525452 (1985-06-01), Jones et al.
Hautanen, A., and E. Linder, "C3c-Binding ELISA for the Detection of Immunoconglutinins and Immunoglobulin Aggregates", In Methods in Enzymology. vol. 74, Part C. (Langone, ed) 1981, pp. 600-601.
Qualitiere et al., J. Immunology, vol. 119 (5), pp. 1645-1651 (1977).
Porstmann et al., Clinica Chimica Acta, vol. 109, pp. 175-181 (1981).

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