Use of plasma to immobilize protein on polymeric surfaces

Details Use of plasma to immobilize protein on polymeric surfaces Use of plasma to immobilize protein on polymeric surfaces

1991-04-18

1992-12-15

Nutter, Nathan M.

Synthetic resins or natural rubbers -- part of the class 520 ser

Synthetic resins

Mixing of two or more solid polymers; mixing of solid...

435180, 435188, 530402, 530815, 436528, 436529, 436530, 436531, G01N 33544, G01N 33545, C12N 1108, C12N 996

Patent

active

051717794

ABSTRACT:
The present invention relates to a method of immobilizing proteins on a polymeric matrix by means of plasma activation and an apparatus and process for the use of such material. The protein mixture is applied to the surface of the polymeric matrix with or without the addition of a crosslinking agent. It is then placed into a plasma generator, wherein the functional groups on both the protein and the matrix molecules are activated to form free radicals. Upon returning from their high energy state, the free radicals form covalent bonds between the proteins and between the protein and the polymeric matrix. Using this method, the proteins are nonspecifically immobilized on the surface of the polymeric matrix. The method can be utilized to immobilize proteins on the surfaces of polymeric membranes, polymeric beads, polymeric tubes and polymeric plates. The immobilized protein has high biological activity and stability.

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Hsiue et al., "Immobilization of Antibody on Porous Membrane for Insulin Enzyme Immunosensor", (Proc. Natl. Sci. Counc. ROC(A)), vol. 11, No. 6, 1987, pp. 483-487.

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