Use of phenols and anilines to increase the rate of peroxidase c

Details Use of phenols and anilines to increase the rate of peroxidase c Use of phenols and anilines to increase the rate of peroxidase c

1986-07-10

1989-05-09

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 10, 435 11, 435 14, 435 17, 435 25, 435 28, 430223, 422 56, C12Q 128, G01N 3358, G01N 3352, G01N 3353

Patent

active

048289833

ABSTRACT:
Certain phenols and anilines can be used as electron transfer agents to increase the rate of oxidation of leuco dyes by peroxidase. These phenols and anilines can react with hydrogen peroxide in the presence of peroxidase to provide intermediates which have higher oxidation potentials than the slowly oxidized substrates, e.g. the leuco dyes. These phenols and anilines can be used to advantage in both solution and dry assays of various analytes. They are particularly useful for the determination of an immunologically reactive ligand in an immunoassay.

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