Use of pH dependence for scatter correction in fluorescent metho

Details Use of pH dependence for scatter correction in fluorescent metho Use of pH dependence for scatter correction in fluorescent metho

1992-06-26

1995-02-07

Saunders, David

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436172, 436537, 436800, G01N 33542, G01N 33543

Patent

active

053875273

ABSTRACT:
A method for correcting for light scattering affects obtained from a sample to which a fluorophore has been added. In accordance with the invention, a sample to which a fluorophore has been added is irradiated with light in the adsorption band of the fluorophore such that the fluorophore emits light at a different intensity. By manipulating the pH of the sample, and obtaining both pre- and post-manipulation emission light intensity readings, the value of the reading attributed to "light scattering" can be determined, such that correction of an erroneous fluorescence reading can be obtained.

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