Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1987-08-04
1990-06-12
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 25, 435 28, 436172, 436518, 422 52, 544234, 544237, G01N 33543, G01N 2176, C12Q 128
Patent
active
049332767
ABSTRACT:
A xanthine oxidase enzyme system to provide long lived entities capable of being recognized by a chemiluminescent reagent is disclosed. In the examples provided, a specific binding pair ligand or analyte is coupled with xanthine oxidase, either directly or via a streptavidin bridge. Thereafter the presence of an analyte can be determined by a chemiluminescent emission upon addition of a signal reagent comprising hypoxanthine, iron EDTA complex and luminol dissolved in barbital buffer. The resulting chemiluminescent signal is stable and detectable for many hours after initiation. The chemiluminescent xanthine oxidase system is particularly useful for immunoassays and DNA probe analysis.
REFERENCES:
patent: 4235869 (1980-11-01), Schwarzberg
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4363759 (1982-12-01), Boguslaski et al.
patent: 4375972 (1983-03-01), Forgione et al.
patent: 4376825 (1983-03-01), Rubenstein et al.
patent: 4478817 (1984-10-01), Campbell et al.
patent: 4563417 (1986-01-01), Albarella et al.
patent: 4598044 (1986-07-01), Kricka et al.
patent: 4604364 (1986-08-01), Kosak
patent: 4617261 (1986-10-01), Sheldon, III et al.
patent: 4629690 (1986-12-01), Weng et al.
patent: 4835101 (1989-05-01), Kao et al.
Chemical Abstracts, vol. 97, No. 21 (Nov. 22, 1982), Abstract 97:177149j.
Hodgson et al, "The Mechanism of the Activity-Dependent Luminescence of Xanthine Oxidase," Archives of Biochemistry and Biophysics 172, 202-205 (1976).
Winterbourn et al, "Iron and Xanthine Oxidase Catalyze Formation of an Oxidant Species Distinguishable from OH: Comparison with the Haber-Weiss Reaction," Archives of Biochemistry and Biophysics, v. 244, No. 1, pp. 27-34 (Jan. 1986).
Kricka et al, "Luminescent Immunoassays: New Labels for an Established Technique, " Diagnostic Medicine, (May 1984) pp. 44-52.
Schroeder et al., "Cehmiluminescence Yields and Detection Limits of Some Isoluminol Derivatives in Various Oxidation Systems," Analytical Chemisty, vol. 50, No. 8 (Jul. 1978).
Wilhelm et al., "Variables in Xanthine Oxidase-Initiated Luminol Chemiluminescence: Implications for Chemiluminescence Measurements in Biological Systems," Analytical Biochemistry 158, 201-210 (1986).
Yaxley, "The Amerlite Enhanced Luminescence Immunoassay System," pp. 1-13.
Canberra Industries, Inc.
Spiegel Carol A.
Warden Robert J.
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