Use of one-dimensional nuclear magnetic resonance to identify li

Details Use of one-dimensional nuclear magnetic resonance to identify li Use of one-dimensional nuclear magnetic resonance to identify li

1997-04-18

2000-03-28

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

422 50, 422 62, 422 681, 422 69, 422 8201, 435 5, 435 6, 435 71, 4352861, 4352871, 4352872, 4352891, 435810, 436501, 935 77, 935 78, 935 88, C12Q 168

Patent

active

060430249

ABSTRACT:
The present invention provides a process for identifying compounds which bind to a specific target molecule. The process comprises the steps of: a) generating a first T.sub.2 - or diffusion-filtered proton spectrum of one or a mixture of chemical compounds; b) exposing one or a mixture of chemical compounds to the target molecule; c) generating a second T.sub.2 - or diffusion-filtered proton spectrum of one or a mixture of chemical compounds that has been exposed the target molecule in step (b); and d) comparing said first and second T.sub.2 - or diffusion-filtered proton spectra to determine differences between said first and said second spectra, the differences identifying the presence of one or more compounds that are ligands which have bound to the target molecule.

REFERENCES:
patent: 5698401 (1997-12-01), Fesik et al.
patent: 5804390 (1998-09-01), Fesik et al.
M. Liu et al., "High Resolution Diffusion and Relaxation Edited One-and Two-Dimensional 1 H NMR Spectroscopy of Biological Fluids", Anal.Chem., vol. 68 (No. 19), p. 3370-3376 (1996).
M. Lin et al., "Mixture Analysis in Combinatorial Chemistry. Application of Diffusion-Resolved NMR Spectroscopy", J. Org. Chem, Vol. 61 (No. 21), p. 7617-7619, (1996).
C. Rossi et al., "Nuclear magnetic resonance as a tool for the identification of specific DNA-ligand interaction", Chemical Physics Letters, vol. 189 (No. 3), p. 278-280, (1992).
S. Meiboom et al., "Modified Spin-Echo Method for Measuring Nuclear Relaxation Times", The Review of Scientific Instruments, Vol. 29, No. 8, (1958) 688-691.
T.M. Logan et al., "Structural Characterization of the FK506 Binding Protein Unfolded in Urea and Guanidine Hydrochloride," Journal of Molecular Biology, Vol. 236, (1994) 637-648.
A.S. Altieri et al., "Association of Biomolecular Systems Via Pulsed Field Gradient NMR Self-Diffusion Measurments," Journal of the American Chemical Society, Vol. 117, (1995) 7566-7567.
S.B. Shuker et al., "Discovering High-Affinity Ligands for Proteins: SAR by NMR," Science, Vol. 274, (1996) 1531-1534.
S.J. Gibbs et al., "A PFG NMR Experiment for Accurate Diffusion and Flow Studies in the Presence of Eddy Currents," Journal of Magnetic Resonance, Vol. 93, (1991) 395-402.

Affiliated with

Also associated with

Rating

Use of one-dimensional nuclear magnetic resonance to identify li does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Use of one-dimensional nuclear magnetic resonance to identify li, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Use of one-dimensional nuclear magnetic resonance to identify li will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1324921