Use of nucleic acids bound to carrier macromolecules

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S006120, C435S091100, C536S023100, C536S024300, C536S024330, C536S025300, C536S025320

Reexamination Certificate

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09760819

ABSTRACT:
A nucleic acid primer is bound to a soluble carrier macromolecule, a multitude of primer molecules thus being carried by each carrier macromolecule, and hybridisation of the primer to a template followed by extension of the primer to replicate the template in complementary form is carried out as part of a PCR procedure or other amplification, or to form an extended primer of greater hybridisation affinity. A second primer used in the amplification may also be bound to a carrier macromolecule.

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Attachment for fluorescein.
Attachment for dextran.
Attachment for lysine and polylysine.
Attachment for alkaline phosphatase.

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