Use of novel L-glutamic acid oxidase

Details Use of novel L-glutamic acid oxidase Use of novel L-glutamic acid oxidase

1983-06-28

1986-09-30

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435191, 435288, 435291, 435810, 435817, C12Q 126, C12N 906, C12M 140, C12M 134

Patent

active

046147144

ABSTRACT:
The present invention consists of an analytical method for assay of L-glutamic acid in a sample by the use of an L-glutamic acid oxidase which is an L-amino acid oxidase catalyzing the oxidative deamination of the .alpha.-amino group of L-glutamic acid in the presence of water and oxygen to form .alpha.-ketoglutaric acid, ammonia and hydrogen peroxide, and having a very high substrate specificity for L-glutamic acid substantially without acting on L-glutamine and L-histidine, and also having a high stability, a reagent for analysis to practice the analytical method, a kit for analysis comprising the reagent, and a biosensor employing the enzyme.

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