Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1997-02-04
1998-09-01
Leary, Louise N.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435 4, 435975, 435 18, 435 21, 435 12, 435 14, 435 26, 435 17, 536 276, 536 2626, 536 267, C12Q 148, C12Q 100, C12Q 134, C12Q 142
Patent
active
058010068
ABSTRACT:
Kits and methods for measuring enzyme activities and metabolites using NADH and NADPH analogs are disclosed. The analogs have extended stability in aqueous solutions and can used as replacements for NADH or NADPH cofactors in analytical procedures. Preferred aspects of the invention include kits containing the NADH and NADPH analogs for use in the measurement of ALT activity, AST activity, Urea, Ammonia, Salicylate, Triglycerides, Pyruvic Acid, Sorbitol Dehydrogenase activity, 5'-Nucleotidase activity, Creatine Kinase activity, 2,3-Diphosphoglyceric Acid, Adenosine 5'-triphosphate, .alpha.-Hydroxybutyrate Dehydrogenase activity, Lactate Dehydrogenase activity and the Carbon Dioxide content in analytical samples.
REFERENCES:
patent: 4250254 (1981-02-01), Modrovich
patent: 4271264 (1981-06-01), Modrovich
patent: 4372874 (1983-02-01), Modrovich
patent: 4394449 (1983-07-01), Modrovich
patent: 5037738 (1991-08-01), Lamos et al.
patent: 5116728 (1992-05-01), Crowther et al.
patent: 5278044 (1994-01-01), San George et al.
patent: 5589348 (1996-12-01), Kaufman et al.
Woenckhaus, C. et al "Preparation and Properties of NAD and NADP Analogs"; Pyridine Nucleotide Enzymes (Co-Enzymes and Co-Factors, vol. III) pp. 449-568 (1987) Dolphin et al, eds.
Stein, A. et al "The Thionicotinamide Analogs of DPN and TPN"; Biochemistry, vol. 2, No. 5, pp. 1015-1022 (Sep.-Oct. 1963).
Alivisatos, S. "Non-Enzymatic Interactions of Reduced Coenzyme I with Inorganic Phosphate and Certain Other Aninos"; Nature, No. 4948, pp. 973-975 (1964).
Kaplan, N., "the 3-Acetylpyridine Analog of DPN"; McCollum-Pratt Institute, No. 76, pp. 1713-1714 (1954).
Alivisatos, S., "Spontaneous Reactions of 1,3-Substituted 1,4-Dihydropyridines with Acids in Water at Neutrality . . . "; Biochemistry, vol. 4, No. 12, pp. 2616-2630 (1965).
Wu, J., "Stability of NADPH: Effect of Various Factory on the Kinetics of Degradation"; Clinical Chemistry, vol. 32/2, pp. 314-319 (1968).
Zatman, L., "Effect of Isonicotinic Acid Hydrazides in Diphosphopyridine Nucleotidases"; J. Biol. Chem. No. 209, pp. 453-465 (1954).
Zatman, L., "The Isolation and Properties of the Isonitinic Acid Hydrazide Analogue of Diphosphopyridine Nucleotide"; J. Biol. Chem., No. 209, pp. 467-484 (1954).
Kaplan, N. et al "Reaction of Pyridine Nucleotide Analogues with Dehydrogenases"; J. Biol. Chem., No. 221, pp. 833-844 (1956).
Lowry, O. et al "The Stability of Pyridine Nucleotides"; J. Bio. Chem., vol. 236, No. 10, pp. 2756-2759 (1961).
Siegel, J. et al "Ultraviolet Absorption Sepctra of DPN and Analogs of DPN"; Archives of Biochemistry and Biophysics, No. 82, pp. 288-299 (1959).
Kaplan, N. et al "Chemistry and Properties of the 3-Acetylpyridine Analogue of Diphosphopyridine Nucleotide"; J. Biol. Chem., No. 221, pp. 823-832 (1956).
Leary Louise N.
Specialty Assays, Inc.
LandOfFree
Use of NADPH and NADH analogs in the measurement of enzyme activ does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Use of NADPH and NADH analogs in the measurement of enzyme activ, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Use of NADPH and NADH analogs in the measurement of enzyme activ will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-269078