Use of N-acetylcysteine to store red blood cells and a method of

Details Use of N-acetylcysteine to store red blood cells and a method of Use of N-acetylcysteine to store red blood cells and a method of

1997-10-22

2000-02-01

Saucier, Sandra E.

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

424 9373, 514562, A01N 102, A01N 6300, A01N 3712

Patent

active

060201207

ABSTRACT:
The invention provides a method of preserving red blood cells. The method comprises treating the red blood cells with about 2 mM to about 35 mM of N-acetylcysteine. The invention further provides a method of aging red blood cells. This method comprises isolating red blood cells; incubating the isolated red blood cells at about 37.degree. C.; and cyclically exposing the incubated red blood cells to N.sub.2 for about 15 minutes then to ambient air for about 5 minutes for a total of about 16 hours. This method allows for the timely screening of agents for their ability to affect the premature aging of red blood cells. This timely screening can be conducted in the course of 16 hours as opposed to weeks or months that would be necessary if the red cells could not be prematurely aged in accordance with the method of the subject invention.

REFERENCES:
Horinaka et al., "Protection of Cellular alpha Tocopherol by Intracellular Glutathione in Human Erythrocytes", Dokkyo J. Med. Sci. 19 (2) : 75-85 (1992).
Runge-Morris et al., "Differential Effects of Organic Hydroperoxides and Hydrogen Peroxide on Proteolysis in Human Erythrocytes", Chemical Research in Toxiodolgy 2 (2) : 76-83 (1989).
Spooner et al., "Heat Stability and Kinetic Properties of Human Serum Glutathione Reductase Activity in Various Disease States", Biochem. Med. 26 (2) : 239-48 (1981).
Mazor et al., "Red Blood Cell Permeability to Thiol Compounds", Eur. J. Haemoatol. 57 (3) : 241-246 (1996).
Lachant et al., "Antioxidant Metabolism during Blood Storage and Its Relationship to Postrransfussion Red Cell Survival", American J. Hematology 17 (3) : 237-49 (1984).
Knight and Searles, Annals of Clinical and Laboratory Science 24:294-301 (1994).

Affiliated with

Also associated with

Rating

Use of N-acetylcysteine to store red blood cells and a method of does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Use of N-acetylcysteine to store red blood cells and a method of, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Use of N-acetylcysteine to store red blood cells and a method of will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-936174