Use of MDCK cell line to predict corneal penetration of drugs

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S007100, C435S032000, C435S040510

Reexamination Certificate

active

11114649

ABSTRACT:
A new method of evaluating the ability of drug molecules to penetrate the cornea is described. The permeation rate of the drug molecules in MDCK cells is utilized to predict the ability of the molecules to penetrate the cornea. The method is useful for in vitro screening of potential new ophthalmic drugs, as well as in the design of new drug molecules for topical ocular administration.

REFERENCES:
patent: 2003/0144336 (2003-07-01), Chen et al.
patent: 2003/0235580 (2003-12-01), Zhang
Dey S, et al. (2003) Molecular evidence and functional expression of P-glycoprotein (MDR1) in human rabbut cornea nad corneal epithelial cell lines. Investigative Ophthalmology and Visual Science vol. 44 No. 7: pp. 2909-2918.
Guo A, et al. (2002) Delineating the contribution of secretory transporters in the efflux of etoposide using Madin-Darby canine kidney cells . . . Drug Metabolism and Disposition vol. 30 No. 4: pp. 457-463.
Botham P, et al. (1997) IRAG working group 3: Cell function-based assays. Food and Chemical Toxicology vol. 35: pp. 67-77.
Anand BS, Patel J, and Mitra AK (2003) Interactions of the Dipeptide Ester Produrgs of Acyclovir with the intestinal oligopeptide transporter: competitive inhibition of glycylsarcosine transport in human intestinal cell line Caco-2. J Pharmacol Exp Therapeut 304: pp. 781-791.
Putnam WS, Pan L, Tsutsui K, Takahashi L, Benet LZ (2002) Comparison of bidirectional transport across MDCK and Caco-2 cell monolayers: interactions with peptide transporters. Pharmaceut Res 19: pp. 27-33.
Shen, et al., “Evaluation of an Immortalized Retinal Endothelial Clel Line as an in Vitro Model for Drug Transport Studies Across the Blood-Retinal Barrier”;Pharmaceutical Research; vol. 20, No. 9, pp. 1357-1363 (2003).
Edwards, et al., “Predicted Permeability of the Corneal to Topical Drugs”,Pharmaceutical Research; vol. 18, No. 1; pp. 1497-1508.
Malhotra, et al., “Permeation through Cornea”,Indian Journal of Experimental Biology, vol. 39, pp. 11-24; Jan. 2001.
Sasaki, et al., “Intestinal Permeability of Ophthamlic B-Blockers for Predicting Ocular Permeability”,Journal of Pharmaceutical Sciences; vol. 83, No. 9; pp. 1335-1338; (Sep. 1994).
Ranta, et al., “Ocular Pharmacokinetic Modeling Using Corneal Absorptin and Desorption Rates from in Vitro Permeating Experiments with Cultured Corneal Epithelial Cells”,Pharmaceutical Research, vol. 20, No. 9, pp. 1409-1416 (Sep. 2003).
Yoshida, et al., “Unified Model for the Corneal Permeability of Related and Diverse Compounds with Respect to Their Physicochemical Properties”,Journal of Pharmaceutical Sciences, vol. 85, No. 8, pp. 819-823; (Aug. 1996).
Wang, et al., “Lipophilicity Influence on Conjunctival Drug Penetration in the Pigmented Rabbit: A Comparison with Corneal Penetration”,Current Eye Research, vol. 10, No. 6, pp. 571-579, (1991).
Liu, et al., “Pharmacokinetics of Sparfloxacin in the Serum and Vitreous Humor of Rabbits: Physicochemical Properties That Regulate Penetration of Quinolone Antimicrobials”,Antimicrobial Agents and Chemotherapy, vol. 42, No. 6, pp. 1417-1423; (Jun. 1998).
Prausnitz, et al., “Permeability of Cornea, Sclera, and Conjuctiva: A Literature Analysis for Drug Delivery to the Eye”,Journal of Pharmaceutical Sciences, vol. 87, No. 12, pp. 1479-1488; (Dec. 1998).
Fukuda, et al., “In Vitro Topically Applied Fluoroquinolone Penetration into the Anterior Chamber”,Journal of the Japanese Ophthalmic Society, vol. 99, pp. 532-536, (1995).
Viswanadhan, et al., “Atomic Physicochemical Parameters for Three Dimensional Structure Directed Quantative Structure-Activity Relationshps”,J. Chem. Inf. Computer Science, vol. 29, pp. 163-172 (1989).
Kowalski, et al., “Gatifloxacin and Moxifloxacin: An In Vitro Susceptibility Comparison to Levofloxacin, Ciprofloxacin, and Ofloxacin Using Bacterial Keratitis Isolates”,American Journal of Opthalmology, vol. 136, No. 3, pp. 500-505; (Sep. 2003).
Mather, et al., “Fourth Generation Fluoroquinolones: New Weapons in the Arsenal of Ophthalmic Antibiotics”;American Journal of Ophthalmology, vol. 133, No. 4, pp. 463-466, (Apr. 2002).
Pestova, et al., “Intracellular Targets of Moxifloxacin: a Comparison With Other Fluoroquinolones”,British Society of Antimicrobial Chemotherapy, vol. 45, pp. 583-590; (2000).
Jester, et al., “Area and Depth of Surfactant-Induced Corneal Injury Predicts Extent of Subsequent Ocular Responses”,Investigative Ophthalmology&Visual Science, vol. 39, No. 13, pp. 2610-2625, (Dec. 1998).
Horio, et al., “Transepithelial Transport of Vinblastine by Kidney-Derived Cell Lines. Application of a New Kinetic Model to Estimate in situ Kmof the Pump”,Biochimica et Biophysica Acta, pp. 116-122, (1990).
Pan, et al., “Enhanced Transepithelial Flux of Cimetidine by Madin-Darby Canine Kidney Cells Overexpressiing Human P-Glycoprotein”,Journal of Pharmacology and Experiemental Therapeutics,vol. 270, No. 1, pp. 1-7, (1994).
Arimori, et al., “Effect of P-Glycoprotein Modulator, Cyclosporin A, on the Gastrointestinal Excretion of Irinotecan and its Metabolite SN-38 in Rats”,Pharmaceutical Research, vol. 20, No. 6, pp. 910-916.
Lee, et al., “Improved Ocular Drug Delivery With Prodrugs in Prodrugs”,Topical and Ocular Drug Delivery, Sloan, K.B., (ed.), pp. 221-297, Marcel Dekker, NY, NY, (1992).
Schoenwald, et al., “Relationshp Between Steroid Permeability Across Excised Rabbit Cornea and Octanol-Water Partition Coefficients”Journal of Pharmaceutical Sciences, vol. 67, No. 6, pp. 786-788; (Jun. 1978).
Schoenwald, et al. “Corneal Penetration Behavior of B-Blocking Agents I: Physicochemical Factors”,Jounral of Pharmaceutical Sciences, vol. 72, No. 11, (Nov. 1983).
Wu, et al, “In-Vitro Corneal Permeability of Cephalosporins”,The Chinese Pharmaceutical Journal, vol. 43, No. 3, pp. 229-235, (Feb. 1991).
Ruiz-Garcia, et al., “Biophysical Models in Drug Development: 6-Fluoroquinolone Derivatives”,Proceedingsd of the International Symposium on Controlled Release of Bioactive Materials, 27thEdition, pp. 708, (2000).
Mariscal, et al., “Tight Junction Formation in Cultured Epithelial Cells (MDCK)”,Journal of Membrane Biology, vol. 86, pp. 113-125, (1985).
Fu, et al., “Prediction of Corneal Permeability Using Polar Molecular Surface Areas”,Pharmazie, vol. 56, No. 8, p. 667, (2001).
Fu, et al., “A Simple Model for the Prediction of Corneal Pemeability”,International Journal of Pharmaceutics, vol. 232, pp. 193-197, (2002).
Clark, “Rapid Calculation of Polar Molecular Surface Area and Its Application to the Prediction of Transport Phenomea. 1. Prediction of Intestinal Absorption”,journal of Pharmaceutical Sciences, vol. 88, No. 8, pp. 807-814, (Aug. 1999).
Toropainen, et al., “Paracellular and Passive Transcellular Permeability in Immortalized Human Corneal Epithelial Cell Culture Model”,European Journal of Pharmaceutical Sciences, vol. 20, pp. 99-106; (2003).
Romanowski, et al., “The in vitro e valuation of the ophthalmic fluoroquinolones against bacterial conjunctivities isolates”,Presented at the Ocular Microbiology and Immunology Group Meeting, Anaheim, CA, Nov. 15, 2003, Abstract 1.
Caballero, et al., “Effectiveness of fluoroquinolone antibiotics for experimental mycobacterium chelonae keratitis”,Presented at the Ocular Microbiology and Immunology Group Meeting, Anaheim, CA, Nov. 15, 2003, Abstract 22.
Katz, et al., “Human aqueous humor penetration pharmacokinetics of moxifloxacin afte topical administration of moxifloxacin 0.5% ophthalmic solution”,Presented at the Ocular Microbiology and Immulology Group Meeting, Anaheim, CA, Nov. 15, 2003, Abstract 10.
Kim, et al., “Evaluation of the effects of topical ophthalmic fluoroquinolones (FQ) on the cornea using in v

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Use of MDCK cell line to predict corneal penetration of drugs does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Use of MDCK cell line to predict corneal penetration of drugs, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Use of MDCK cell line to predict corneal penetration of drugs will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3819646

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.