Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1998-08-13
1999-09-07
Leary, Louise N.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435 34, 435847, 435848, 435849, 436 71, 436 13, 436173, C12Q 100, C12Q 104, G01N 3392
Patent
active
059486104
ABSTRACT:
The present invention is directed to a method of using a liquid matrix containing a black body light absorbing powder to facilitate the analysis of biomarkers from representative microorganisms by laser desorption mass spectrometry. Both an IR laser (1064 nm) and a UV laser (337 nm) were shown to be compatible and both time-of-flight and Fourier-transform mass analyzer were used. In the present implementation gram negative and gram positive bacteria were suspended in a methanol:chloroform solution and added to a cobalt/glycerol matrix, S/N, sensitivity and sampling time are greatly enhanced for polar lipid biomarkers.
REFERENCES:
patent: 5059527 (1991-10-01), White et al.
Bargiota et al; Int. J. Food Microb; vol. 4(3) pp. 257-266; (1987) (Abstract) Month Not Available.
Fenselau Catherine
Ho Yen-Peng
Adler Benjamin Aaron
Leary Louise N.
University of Maryland at Baltimore County
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