Use of ionic liquids for protein extraction

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification

Reexamination Certificate

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C530S422000

Reexamination Certificate

active

07470778

ABSTRACT:
The present invention relates to a method for the extraction of proteins, protein fragments and/or peptides from biological samples, where the extractants employed are ionic liquids of the general formula K+A−, to a kit for the extraction of proteins, protein fragments and/or peptides, and to the use thereof.

REFERENCES:
patent: 2006/0149035 (2006-07-01), Rudolph et al.
patent: 10240098 (2004-03-01), None
patent: WO 2004/024279 (2004-03-01), None
patent: WO 2004/080579 (2004-09-01), None
patent: WO 2004/086001 (2004-10-01), None
patent: WO 2005/103070 (2005-11-01), None
Shimojo, K., et al. 2006 Biomacromolecules 7(1): 2-5, (plus chart 1).
Jain, N. et al., “Chemical and biochemical transformations in ionic liquids,” Tetrahedron, vol. 61 No. 5, Jan. 31, 2005, Elsevier Science Publishers, Amsterdam, NL, pp. 1015-1060.
Yang, Z. et al., “Ionic liquids: Green solvents for nonaqueous biocatalysis,” Enzyme and Microbial Technology, vol. 37 No. 1, Jun. 1, 2005, Stoneham, MA, USA, pp. 19-28.

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