Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1991-11-14
1993-05-18
Griffin, Ronald W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 14, 435 27, 435 10, 435805, C12Q 154, C12Q 128
Patent
active
052120660
ABSTRACT:
Disclosed is a novel diagnostic reagent system for the detection of hydroperoxides or substances which react with peroxidatively active substances resulting in the liberation of hydroperoxides which reagent system comprises a chromogenic oxidation indicator and a peroxidase or a peroxidatively active substance, together with an inhibitor of color generation selected from the group of 1,4-disubstituted semicarbazides, thiosemicarbazides and 1,5-disubstituted carbazides as inhibitors of color generation. By inhibiting the generation of color formed by oxidation of the chromogenic indicator, the indicator's usefulness in discriminating between varying concentrations of analyte is enhanced.
REFERENCES:
patent: 4220713 (1980-09-01), Rittersdorf et al.
G. R. Schonbaum, J. Biol. Chem. 248 (1973) 502-511 "New Complexes of Peroxidases with Hydroxamic Acids, Hydrazides, and Amides".
Hidaka, H. et al. Arch. Biochem. Biophys. 140, (1970) 174-180--"Evidence of a Hydrazine-Reactive Group at the Active Site of the Nonheme Portion of Horseradish Peroxidase".
Morrison et al; "Organic Chemistry"; 3rd Edition; pp. 366-367 (1973).
Usmani et al; "Chemical Abstracts"; 112(19):17524p (1989).
Albarella James P.
Cast Todd K.
Michaels David W.
Strycker Wallace G.
Griffin Ronald W.
Jeffers Jerome L.
Leary Louise N.
Miles Inc.
LandOfFree
Use of inhibitors of color generation in chromogenic assays does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Use of inhibitors of color generation in chromogenic assays, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Use of inhibitors of color generation in chromogenic assays will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-803694