Use of immunoglogulin-binding artificial proteins as molecular w

Details Use of immunoglogulin-binding artificial proteins as molecular w Use of immunoglogulin-binding artificial proteins as molecular w

1995-03-20

1996-12-03

Fitzgerald, David L.

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Protein or peptide standard or control

436528, 530350, 435 691, G01N 3368, G01N 33544

Patent

active

055807887

ABSTRACT:
The present invention relates to immunoglobulin-binding artificial proteins (repetitively linked-proteins) which result when either one of or several numbers of the IgG-binding domains in protein A molecule is defined as a unit, and repetitive multiple links are made thereof. These repetitive proteins exhibit a superior ability for IgG purification to naturally occurring protein A, and the mixture of these repetitive proteins may be used as excellent molecular weight markers.

REFERENCES:
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Strandberg, L., et al. (1990) J. Biotechnol. 13:83-96.
Mori, T., et al. (1989) Chem. Abs. 112(9):71653g [English Summary of JP 01060388].
Ljungberg, U. K., et al. (1993) Mol. Immunol. 30:1279-85.
M. Uhlen et al.: "Complete sequence of the Staphylococcal Gene Encoding Protein A", Journal of Biological Chemistry, vol. 259, No. 3, (1984), pp. 1695-1702.
B. Nilsson et al.; "A synthetic IgG-binding Domain Based On Staphylococcal Protein A", Protein Engineering, vol. 1, No. 2, 1987, pp. 107-113.
A. Saito et al.; "High Level Expression Of A Stynthetic Gene Coding for Ig-Biding Domain B of Staphylococcal Protein A", Protein Engineering, vol. 2, No. 6, 1989, pp. 481-487.
Saburo Mitsui et al.; "Dictionary of Biotechnology", CMC Inc. 1986-10-9, p. 958.

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