Use of immobilized mismatch binding protein for detection of mut

Details Use of immobilized mismatch binding protein for detection of mut Use of immobilized mismatch binding protein for detection of mut

1995-04-28

2000-09-05

Myers, Carla J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, C12Q 168, C12P 1934

Patent

active

061141151

ABSTRACT:
A method for detecting mutations, such as a single base change or an addition or deletion of about one to four base pairs, is based on the use of an immobilized DNA mismatch-binding protein, such as MutS, which binds to a nucleic acid hybrid having a single base mismatch or unpaired base or bases, thereby allowing the detection of mutations involving as little as one base change in a nucleotide sequence. Such a method is useful for diagnosing a variety of important disease states or susceptibilities, including the presence of a mutated oncogene and the presence of DNA containing triplet repeat sequences which characterize several genetic diseases including fragile X syndrome. The present method is used to isolate or remove by affinity chromatography duplex DNA molecules containing mismatches such as error-containing molecules in PCR-amplified DNA samples. Also provided are compositions and kits useful for practicing the methods of the present invention.

REFERENCES:
patent: 5556750 (1996-09-01), Modrich
patent: 5750335 (1998-05-01), Gifford
Lishamskaya et al. Am J Hum Genet 51:A385 (Oct. 1992).
Nelson et al. Nature Genetics 4:11-19 (May 1993).
Jiricny, J. et al., "Mismatch-containing oligonucleotide duplexes bound by the E.coli mutS-encoded Protein", Nucl. Acids Res. 16:7843-7853 (1988).
Su, S.S. et al., "Mispair Specificity of Methyl-directed DNA Mismatch Correction in Vitro", J. Biol. Chem. 263:6829-6835 (1988).
Lu, A.S. et al., "Detection of Single DNA Base Mutations with Mismatch Repair Enzymes", Genomics 14:249-255 (1992).
Lishanskaya, A. et al. (Human Genet. 51 (4 Suppl):A385, abstract #1517 (1992).
Lishanski, A. et al., "Mutation detection by mismatch binding protein, MutS, in amplified DNA:Application to the cystic fibrosis gene", Proc. Natl. Acad. Sci. USA 91:2674-2678 (1994 Mar.).
Modrich, P., "Methyl-directed DNA Mismatch Correction", J. Biol. Chem. 264:6597-6600 (1989).
Fazakerley et al., "Structures of mismatched base pairs in DNA and their recognition by the Escherichia coli mismatch repair system", EMBO J. 5:3697-3703 (1986).
Dohet, C. et al., "Large non-homology in heteroduplex DNA is processed differently than single base pair mismatches", Mol. Gen. Genet. 206:181-184 (1987).
Jones, M. et al., "Repair of a Mismatch Is Influenced by the Base Composition of the Surrounding Nucleotide Sequence", Genetics 115:605-610 (1987).
Lu, A.L. et al., "Repair of Single Base-Pair Transversion Mismatches of Escherichia coli in Vitro: Correction of Certain A/G Mismatches Is Independent of dam Methylation and Host mutHLS Gene Functions", Genetics 118:593-600 (1988).
Haber L.T. et al., "Nucleotide Sequence of the Salmonella typhimurium mutS Gene Required for Mismatch Repair: Homology of MutS and HexA of Streptococcus pneumoniae", J. Bacteriol. 170:197-202 (1988).
Pang, P.P. et al., "Identification and Characterization of the mutL and mutS Gene Products of Salmonella typhimurium LT2", J. Bacteriol. 163:1007-1015 (1985).
Priebe S.D. et al., "Nucleotide Sequence of the hexA Gene for DNA Mismatch Repair in Streptococcus pneumoniae and Homology of hexA to mutS of Escherichia coli and Salmonella typhimurium", J. Bacteriol. 170:190-196 (1988).
Valle G. et al., "The Sequence of a 6.3 kb Segment of Yeast Chromosome III Reveals an Open Reading Frame Coding for a Putative Mismatch Binding Protein", 1991 Yeast 7:981-988.
Miret J.J. et al., "Characterization of a DNA Mismatch-binding Activity in Yeast Extracts", 1993, J. Biol Chem. 268:3507-3513.
Stephenson, C. et al., "Selective Binding to DNA Base Pair Mismatches by Proteins from Human Cells", 1989, J. Biol. Chem. 264:21177-21782.
Karran, P. et al., "Mismatch binding proteins and tolerance to alkylating agents in human cells", 1990, Mutat. Res. 236:269-275.
Hughes M.J. et al., "The Purification of a Human Mismatch-binding Protein and Identification of Its Associated ATPase and Helicase Activities", 1992, J. Biol. Chem. 267:23876-23882.
Reenan, A.G. et al., "Isolation and Characterization of Two Saccharomyces cerevisiae Genes Encoding Homologs of the Bacterial HexA and MutS Mismatch Repair Proteins", 1993, Genetics 132:963-973.
Reenan, A.G. et al. "Characterization of Insertion Mutations in the Saccharomyces cerevisiae MSH1 and MSH2 Genes: Evidence for Separate Mitochondrial and Nuclear Functions", 1993, Genetics 132:975-985.
Fishel, R. et al., "Purified Human MSH2 Protein Binds to DNA Containing Mismatched Ducleotides", Cancer Res. 54:5539-5542 (Nov. 1, 1994).
Fishel, R. et al., "Binding of Mismatched Microsatellite DNA Sequences by the Human MSH2 Protein", Science 266:1403-1405 (Nov. 25, 1994).
Fishel, R. et al., "The Human Mutator Gene Homolog MSH2 and Its Association with Hereditary Nonpolyposis Colon Cancer", Cell 75:1027-1029 (1993).
Leach, S.S. et al., "Mutations of a mutS Homolog in Hereditary Nonpolyposis Colorectal Cancer", Cell 75:1212-1225 (1993).
New et al., "The Yeast gene MSH3 defines a new class of eularyotic MutS homologues", Mol. Gen. Genet. 239:97-108 (1993).
Shimada, T. et al., "A 165-Base Pair Sequence Between the Dihydrofolate Reductase Gene and the Divergently Transcribed Upstream Gene is Sufficient for Bidirectional Transcriptional Activity", J. Biol. Chem. 264: (1989) p. 20171-20174.
Linton, J. et al., "Dual Bidirectional Promoters at the Mouse dhfr Locus: Cloning and Characterization of Two mRNA Classes of the Divergently Transcribed Rep-1 Gene", Molec. Cell. Biol. 7:3058-3072 (1989).
Fujii, H. et al., "Isolation and Characterization of cDNA Clones Derived from the Divergently Transcribed Gene in the Region Upstream from the Human Dihydrofolate Reductase Gene", J. Biol. Chem. 264:10057 (1989) p. 10057-10064.
Bowen, B. et al., "The Detection of DNA-Binding Proteins by Protein Blotting", Nucl. Acids Res. 8:1-20 (1979).
Miskimins, W.K. et al., "Use of a Protein-Blotting Procedure and a Specific DNA Probe to Identify Nuclear Proteins that Recognize the Promoter Region of the Transferrin Receptor Gene", Proc. Natl. Acad. Sci. USA 82:6741-6744 (1985).
Keller, A.D. et al., "Selection of Sequences Recognized by a DNA Binding Protein Using a Preparative Southwestern Blot", Nucl. Acids Res. 19:4675-80 (1991).
Norby, P.L. et al., "Determination of Recognition-Sequences for DNA-Binding Proteins by a Polymerase Chain Reaction Assisted Binding Site Selection Method (BSS) Using Nitrocellulose Immobilized DNA Binding Protein", Nucl. Acids Res. 20:6317-6321 (1992).
Singh et al., "Molecular Cloning of an Enhancer Binding Protein: Isolation by Screening of an Expression Library with a Recognition Site DNA", Cell 52:415-423 (1988); Singh et al., Bio Techniques 7:253-261 (1989).
Vinson et al., "In Situ Detection of Sequence-Specific DNA Binding Activity Specified by a Recombinant Bacteriophage", Genes & Devel. 2:801-806 (1988).
Oliphant, A.R. et al., "Defining the Sequence Specificity of DNA-Binding Proteins by Selecting Binding Sites from Random-Sequence Oligonucleotides: Analysis of Yeast GCN4 Protein", Molec. Cell. Biol. 9:2944-2949 (1989).
Perrino, F.W. et al., "Interaction of a Folded Chromosome-Associated Protein With Single-Stranded DNA-Binding Protein of Escherichia coli, Identified by Affinity Chromatography", J. Biol. Chem. 263:11833-11839 (1988).
Meyer, R.R. et al., "The Single-Stranded DNA-Binding Protein of Escherichia coli", Microbiol. Rev. 54:342-380 (1990).
Bhattacharya, N.P. et al., "Mutator phenotypes in human colorectal carcinoma cell lines", Proc. Natl. Acad. Sci. USA 91:6319-6323 (1994).
Bronner, E.C. et al., "Mutation in the DNA mismatch repair gene homologue hMLH 1 is associated with hereditary non-polyposis colon cancer", Nature 368, 258-261 (1994).
Parker, B.O. et al., "Repair of DNA heteroduplexes containing small heterologous sequences in Escherichia coli", Proc. Natl. Acad. Sci. USA 89:1730-1734 (1992).
Alani, E et al., "The Saccharomyces cerevisiae Msh2 protein specifically binds to duplex oligonucleotides containing mismatched DNA base pairs and insertions", Genes Dev. 9:234-247 (Jan. 15, 1995).

Affiliated with

Also associated with

Rating

Use of immobilized mismatch binding protein for detection of mut does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Use of immobilized mismatch binding protein for detection of mut, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Use of immobilized mismatch binding protein for detection of mut will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2210873