Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1984-12-03
1988-05-17
Marantz, Sidney
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
2101982, 210656, 436547, 436548, 436824, 530415, 530416, 530808, A61K 39395, C07K 300, G01N 33577
Patent
active
047451831
ABSTRACT:
A novel method is disclosed for the purification of monoclonal antibodies from antibodies of the same isotype but different light chain composition which are frequently present in the fluids in which such antibodies are generated. According to the method, the mixture is placed in a hydroxylapatite chromatography column and eluted with a buffer solution in a concentration gradient elution. With the appropriate optimization of system parameters, a separation of antibodies effective for both analytical and preparative purposes is obtained.
REFERENCES:
patent: 4469630 (1984-12-01), Flashner
Regnier, F. E., Science, vol. 222(4621), pp. 245-252, 1983.
LKB2204-HA Catalogue, 5/80.
Bio-Rad Catalogue, Chemical Division, Jan. 1984, pp. 104-106, (Chromatography).
Chemical Abstracts, 101:70595a, (1984).
Engelhorn Sheldon C.
Juarez-Salinas Hector
Ott Gary S.
Bio-Rad Laboratories, Inc.
Marantz Sidney
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