Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Reexamination Certificate
2003-09-26
2009-11-10
Steele, Amber D. (Department: 1639)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
C530S350000
Reexamination Certificate
active
07615617
ABSTRACT:
A novel approach is described for reversing aggregation and increasing refolding by application of hydrostatic pressure. A protein of interest in an aggregated, or inclusion body, or other non-native or inactive state is subjected to high hydrostatic pressure. This treatment denatures the protein to states (or conformations) competant for refolding and results in increased formation of native protein once pressure is released. The technique can facilitate conversion non-native proteins, including inclusion bodies and aggregates to native proteins without addition of chaotropic agents, changes in buffer, or large-scale dilution of reagents required for traditional refolding methods.
REFERENCES:
patent: 5288462 (1994-02-01), Carter et al.
patent: 6489450 (2002-12-01), Randolph et al.
patent: 6635469 (2003-10-01), Litt et al.
patent: WO 02/062827 (2002-08-01), None
Lehninger et al., “Principles of Biochemistry with an Extended Discussion of Oxygen-Binding Proteins”, 1993, Worth Publishers, Second Edition, p. 160.
Paladini et al., “Pressure-induced reversible dissociation of enolase”, Apr. 28, 1981, Biochemistry, vol. 20, No. 9, pp. 2587-2593.
Panick et al., “Structural characterization of the pressure-denatured state and unfolding/refolding kinetics of staphylococcal nuclease by synchrotron small-angle X-ray scattering and Fourier-transform infrared spectroscopy”, Jan. 16, 1998, Journal of Molecular Biology, vol. 275, Issue 2, pp. 389-402.
Gorovits et al., “High Hydrostatic Pressure Can Reverse Aggregation of Protein Folding Intermediates and Facilitate Acquisition of Native Structure”, Apr. 28, 1998, Biochemistry, vol. 37, No. 17, pp. 6132- 6135.
Lehninger et al., “Principles of Biochemistry with an Extended Discussion of Oxygen-Binding Proteins”, 1993, Worth Publishers, Second Edition, p. 160.
Foguel et al., 1999, Hydrostatic Pressure Rescues native protein form Aggregates, Biotechnology and Bioengineering, 63(5): 552-558.
Silva et al., 1989, Anomalous Pressure Dissociation of Large Protien Aggregates, The Journal of Biological Chemistry, 264(27): 15863-15868.
Bonafe et al., 1994, Intermediate States of Assembly in the Dissociation of Gastropod Hemocyanin by Hydrostatic Pressure, Biochemistry, 33: 2651-2660.
Anne S. Robinson, Grant Application Abstract, Career: Characterization, Inhibition, and Reversal of Protein Aggregation, Jun. 1, 2000, Abstract Only.
Anne S. Robinson, Grant Application Abstract, Powre: Molecular Determinants and Inhibition of Protein Aggregation, Oct. 1, 1997, Abstract Only.
Anne S. Robinson, Oral Presentation, Engineering Approaches to Reversing Protein Aggregation, Mid-Atlantic Biochemical Engineering Consortium, Apr. 7, 2000, University of Delaware, Abstract, Abstract Only.
Anne S. Robinson, Poster Presentation, The Role of Cysteines and Disulfide Bonds in the Protein Folding of P22 Tailspike, Mid-Atlantic Biochemical Engineering Consortium, Apr. 7, 2000, University of Delaware, Abstract, Abstract Only.
Cleland, “Impact of Protein Folding on Biotechnology”, Protein folding: In vivo and in vitroAmerican Chemical Society(1993) 526: 1-21.
DeBernardez-Clark et al., “Inclusion Bodies and Recovery of Proteins from the Aggregated State”,Protein Refolding, American Chemical Society(1991) 470: 1-20.
Foguel et al., “Characterization of a Partially Folded Monomer of the DNA-binding Domain of Human Papillomavirus E2 Protein Obtained at High Pressure”,J Biol Chem(1998) 273(15): 9050-7.
Gorovits et al., “High Hydrostatic Pressure Can Reverse Aggregatin of Protein Folding Intermediates and Facilitate Acquisition of Native Structure”,Biochemistry(1998) 37(17): 6132-5.
Jurkiewicz et al., “Inactivation of simian immunodeficiency virus by hydrostatic pressure”,Proc. Natl. Acad. Sci. USA(1995) 92: 6935-7.
Robinson et al., “Hydrostatic and Osmotic Pressure as Tools to Study Macromolecular Recognition”,Methods in Enzymol(1995) 259: 395-427.
Shigehisa et al., “Effects of high hydrostatic pressure on characteristics of pork slurries and inactivation of microorganisms associated with meat and meat products”Int J Food Microiol(1991) 12(2-3): 207-15.
Silva et al., “Effects of hydrostatic pressure on a membrane-enveloped virus: High Immunogenicity of the pressure-inactivated virus”,J. Virol.(1992) 66: 2111-7.
Silva et al., “Pressure Stability of Proteins”,Annu. Rev. Phys. Chem.(1993) 44: 89-113.
Silva et al., “The use of hydrostatic pressure as a tool to study viruses and other macromolecular assemblages”,Current Opinion in Structural Biology(1996) 6(2): 166-75.
Tauscher, “Pasteurization of food by hydrostatic high pressure: chemical aspects”Z Lebensm Unters Forsch(1995) 200(1): 3-13.
Gorovits et al., “Rhodanese folding is controlled by the partitioning of its folding intermediates”,Biochimica et Biophysica Acta 1382(1998) 120-128.
Webb et al., “Stability of Subtilisin and Lysozyme under High Hydrostatic Pressure”,American Chemical Society and American Institute of Chemical Engineers(2000) A-G.
Silva et al., “Dissociation of a native dimer to a molten globule monomer. Effects of pressure and dilution on the association equilibrium of arc repressor”,J Mol Biol.(1992) 223(2): 545-55.
Pontes et al., “Pressure Inactivation of Animal Viruses: Potential Biotechnological Applications”,High Pressure Research in the Biosciences and Biotechnology,K. Heremans (Ed.) Leuven University Press, Leuven, Belgium, 1997.
Smelt et al., “Inactivation Kinetics of Microorganisms by High Pressure”,Pressure Research in the Biosciences and Biotechnology, K. Heremans (Ed.) Leuven University Press, Leuven, Belgium, 1997.
Patterson et al., “The Effect of High Hydrostatic Pressure Treatment on Micro-organisms in Foods”,Pressure Research in the Biosciences and Biotechnology, K. Heremans (Ed.) Leuven University Press, Leuven, Belgium, 1997.
Foguel et al., Hydrostatic Pressure Rescues Native Protein from Aggregates,Biotechnology and Bioengineering(1999) 63(5):552-558.
Foguel Debora
Robinson Anne Skaja
Robinson Clifford R.
Silva Jerson Lima
RatnerPrestia
Steele Amber D.
University of Delaware
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