Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1995-02-24
1999-11-23
Wilson, James O.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
424 88, 424 93, 435 29, 4352401, 4352402, 43524021, 43524023, 4352403, 435243, 435245, 4352521, 435253, 435258, 435260, C12Q 102, C12N 500, C12N 100, C12N 136
Patent
active
059898488
ABSTRACT:
This invention provides a purified immortalized human endothelial cell infected with Ehrlichia chaffeensis or Ehrlichia canis. Also provided is a method of simultaneously screening a sample from a human subject for the presence of E. chaffeensis and Rickettsia rickettsii comprising contacting the sample with immortalized human endothelial cells under conditions which allow infection of the cells and detecting the presence of infection, the presence of infection indicating the presence of E. chaffeensis and/or R. rickettsii. The invention also provides a method of screening a sample from a human subject for the presence of E. chaffeensis comprising contacting the sample with endothelial cells under conditions which allow infection of the cells by E. chaffeensis and detecting the presence of infection by E. chaffeensis, the presence of infection by E. chaffeensis indicating the presence of E. chaffeensis in the sample. Finally, the invention provides a method of culturing E. chaffeensis or E. canis comprising contacting E. chaffeensis or E. canis with immortalized human endothelial cells under conditions which allow the propagation of E. chaffeensis or E. canis.
REFERENCES:
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Lawley et al. in Ryan (Ed.) Endothelial Cells vol. III pp. 229-240 CRC Press, (1987).
Kelly et al.American Society for Micro. (1985) Mar. 3-7, 1985 p. 171 Abstract No. J 17.
Wilson. 1991. J. Clin. Microbiol. 29:2838-2842.
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Walker. 1989. Clin. Microbiol. Rev. 2:227-240.
Kelly et al. ASM Annual Mtg (1985) Abstract No. J 17.
Hines Ja-Na A.
The United States of America as represented by the Department of
Wilson James O.
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