Use of heme-containing proteins as stabilizers for enzyme-labele

Details Use of heme-containing proteins as stabilizers for enzyme-labele Use of heme-containing proteins as stabilizers for enzyme-labele

1990-05-11

1993-07-27

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 79, 435 792, 435 28, 435962, 435975, 435 795, 436510, 436512, 436818, 436826, G01N 33571, G01N 3353, G01N 33563

Patent

active

052310043

ABSTRACT:
Heme-containing proteins, such as cytochrome c, are useful in admixture with enzyme-labeled immunoreactants, such as peroxidase-labeled antibodies or fragments thereof. The heme-containing proteins and enzyme-labeled immunoreactants can be supplied in a buffered composition as part of a test kit. The buffered composition comprising the heme-containing protein and peroxidase-labeled immunoreactant excludes 4'-hydroxyacetanilide, which is a phenolic electron transfer agent. The composition can be used in immunoassays for detecting various immunologically reactive species, such as hCG, and chlamydial or gonococcal antigens.

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Vellacott et al., Lancet, 1: pp. 18-19, (Jan. 3, 1981).
Voller et al., "Chapter 17--Enzyme-Linked Immunosorbent Assay", Manual of Clinical Laboratory Immunology, 3rd Ed., Rose et al., editors, pp. 99-109 (1986).

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