Use of green fluorescent protein to trace the infection of bacul

Details Use of green fluorescent protein to trace the infection of bacul Use of green fluorescent protein to trace the infection of bacul

1997-06-17

1999-07-06

Mosher, Mary E.

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Genetically modified micro-organism, cell, or virus

424 936, 435471, 47 581, A01N 6300, C12N 1500

Patent

active

059194456

ABSTRACT:
Green fluorescent protein (GFP) gene was transplanted into baculovirus. The baculovirus was used to infect pests of vegetable crops. Three to five days after infection, the infected insects were found to emit strong fluorescence when exposed to a portable UV light. Therefore the spreading of baculovirus infection and disease progression can be effectively detected in the field. In addition, the GFP reduced UV damage to the baculovirus. As a result, GFP can increase the retention time of the virus as a pesticide in the field.

REFERENCES:
Chao et al. Nature, vol. 380, pp. 396-397, Apr. 4, 1996.
Ericksson et al, Journal of Virological Methods 59: 127-133, May 1996.

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