Use of gel polymer for DNA extraction with organic solvents

Details Use of gel polymer for DNA extraction with organic solvents Use of gel polymer for DNA extraction with organic solvents

1989-07-10

1992-04-21

Griffin, Ronald W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 28, 536 29, 536127, 435 6, 435803, C07H 2100, C07H 2104

Patent

active

051069660

ABSTRACT:
The invention describes a method for extracting DNA from a sample. It involves contacting a sample with a separation reagent which permits differentiated solvation of DNA and protein. By adding a gel polymer, such as a polyester gel polymer to the mixture of sample and separating reagent followed by agitation via, e.g., centrifugation, the DNA and protein are separated, with the gel acting as a block to prevent contamination of the DNA phase by the protein. Higher yields of DNA are obtained as compared to methodologies where the gel is not used. Additionally, the problems associated with the physical contact of the solvents, which are frequently carcinogens, are avoided. Also taught are kits which can be used in connection with the inventive method.

REFERENCES:
patent: 4833239 (1989-05-01), DeBonville et al.
patent: 4921952 (1990-05-01), Longmire et al.
patent: 4923978 (1990-05-01), McCormick
Maniatis et al. (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory pp. 458-460.
Karger et al. (1973) An Introduction to Separation Science, John Wiley & Sons: New York, pp. 381-388.

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