Use of fungal mutants for expression of antibodies

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S069100, C435S254300

Reexamination Certificate

active

07858360

ABSTRACT:
The disclosure relates to a filamentous fungal cell in which an endogenous alkaline protease activity, an endogenous neutral metalloprotease activity, an endogenous serine protease activity, and an endogenous kexin maturase activity have been completely or partially inactivated. Particularly the endogenous alkaline protease activity, the endogenous.neutral metalloprotease activity, the endogenous serine protease activity, and the kexin maturase activity are encoded by the alp, npI, pepC and kexB genes respectively. The filamentous fungal cell is particularly suitable for production of heterologous proteins such as antibodies.

REFERENCES:
patent: 6013452 (2000-01-01), Christensen et al.
patent: 2005/0158825 (2005-07-01), Power et al.
patent: 0574347 (1993-12-01), None
patent: WO 90/00192 (1990-01-01), None
patent: WO 97/22705 (1997-06-01), None
patent: WO 98/12300 (1998-03-01), None
Tatsumi, H., et al., 1989, “A full length cDNA clone for the alkaline protease fromAspergillus oryzae:Structural analysis and expression inSaccharomyces cerevisiae”,Molecular and General Genetics, vol. 219, No. 1, pp. 33-38.
Murakami, K., et al., 1991, “Isolation and characterization of the alkaline protease gene ofAspergillus oryzae”,Agricultural and Biological Chemistry, vol. 55, No. 11, pp. 2807-2811.
Frederick, G. D., et al., 1993, “Cloning and characterisation of pepC, a gene encoding a serine protease fromAspergillus niger”,Gene, vol. 125, No. 1, pp. 57-64.
Jaton-Ogay, K., et al., 1994, “Cloning and disruption of the gene encoding an extracellular metalloprotease ofAspergillus fumigatus”,Molecular Microbiology, vol. 14, No. 5, pp. 917-928.
Yu, C.-J., et al., 1999, “Characterization of a novel allergen, a major IgE-binding protein fromAspergillus flavus,as an aklaline serine protease”, Biochemical and Biophysical Research Communications, vol. 261, pp. 669-675.
Blinkovsky, A. M., et al., 2000, “A non-specific aminopeptidase fromAspergillus”,Biochimica et Biophysica Acta, vol. 1480, pp. 171-181.
Jousson, O., et al., 2004, “Multiplication of an ancestral gene encoding secreted fungalysin preceded species differentiation in the dermatophytes Trichophyton and Microsporum”, Microbiology, vol. 150, pp. 301-310.
Machida, M., et al., 2005, “Genome sequencing and analysis ofAspergillus oryzae”,Nature, vol. 438, No. 22, pp. 1157-1161.
Punt et al., “The role of theAspergillus nigerfurin-type protease gene in processing of fungal proproteins and fusion proteins. Evidence for altemative processing of recombinant (fusion-) proteins”, Journal of Biotechnology, vol. 106, No. 1, pp. 23-32 (2003).
Hombergh Van Den et al., “New protease mutants inAspergillus nigerresult in strongly reducted in vitro degradation of target proteins”, Current Genetics, vol. 28, No. 4, pp. 299-308 (1995).
Hombergh Van Den et al., “Production of the homologous pectin lyase B protein in six genetically defined protease-deficientAspergillus nigermutant strains”, Current Genetics, vol. 32, No. 1, pp. 73-81 (1997).
Frederick et al., “Cloning and characterization of pepC, a gene encoding a Serine protease fromAspergillus niger”,Gene, vol. 125, pp. 57-64 (1993).
Jalving et al., “Characterization of the Kexin-Like Maturase ofAspergillus niger”. Applied and Environmental Microbiology, vol. 66, pp. 363-368 (2000).
Kwon et at., “Molecular cloning of kpcA Gene Encoding Kex2p-like Endoprotease fromAspergillus nidulans”Molecular Cell. vol. 12, pp. 142-147 (2001).
Mizutani et al., “Disorder cell Integrity signaling caused by disruption of the kexB gene inAspergillus oryzae”Eukaryotic Cell, vol. 31, pp. 1036-1048 (2004).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Use of fungal mutants for expression of antibodies does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Use of fungal mutants for expression of antibodies, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Use of fungal mutants for expression of antibodies will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-4204760

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.