Chemistry: analytical and immunological testing – Optical result – With fluorescence or luminescence
Reexamination Certificate
2000-08-21
2003-09-30
Warden, Jill (Department: 1743)
Chemistry: analytical and immunological testing
Optical result
With fluorescence or luminescence
C436S086000, C436S089000, C436S106000, C436S111000
Reexamination Certificate
active
06627455
ABSTRACT:
DESCRIPTION
The present invention relates to the use of fluorescent dyes bearing at least one reactive group capable of reaction with nucleophilic groups for the qualitative and, if desired, quantitative fluorescence-analytical determination of the surface adsorption of compounds bearing amino and/or imino groups.
The most common use for fluorescent dyes is in dyeing/coloring fibers and moldings. However, the property of fluorescence is also utilized in analysis. For instance, fluorescent chromophores are used in biochemistry for labeling antibodies or low molecular weight compounds in immunoassays or other immunological methods of detection. However, in these methods, the fluorescent dyes are coupled to the detecting reagents prior to the detection reaction.
One method for analyzing amine mixtures is described in Anal. Chem. 1995, 67, 1742-1748. Here, amine mixtures are labeled with fluorescent dyes containing a succinimidyl ester radical and separated by liquid chromatography, and the qualitative and quantitative composition of the mixture is determined against previously prepared reference samples.
The determination of the adsorption of amines on surfaces has a different purpose. It is concerned not with the detection of certain amines in mixtures, but with the affinity of an oligo- or polyamine for certain materials.
To develop textile, leather and paper assistants, it is very important to know the degree of adsorption of the oligo- or polyamine when used on the substrates. Examinations into the affinity of specific oligo- and polyamines for the substrates are particularly important when used with other kinds of assistants.
It is an object of the present invention to provide labels providing qualitative and, if desired, quantitative information on oligo- and/or polyamines adsorbed on the substrate.
We have found that this object is achieved by the use of fluorescent dyes bearing at least one reactive group capable of reaction with nucleophilic groups for the qualitative and quantitative fluorescence-analytical determination of the surface adsorption of compounds bearing amino and/or imino groups.
In useful fluorescent dyes, the chemical nature of the chromophore matters only to a minor degree. What is essential, on the contrary, is its reactive group, which forms a rapid bond with any adsorbed compounds having amino and/or imino groups. Fluorescent dyes are known to those skilled in the art and are described for example in Ullmann's Encyclopedia of Industrial Chemistry 5
th
Ed., Vol. A11, p. 279. Useful classes of such dyes are naphthalimides, coumarins, xanthenes, thioxanthenes, naphthilactams, azlactones, methines, stilbenes, oxazines and thiazines.
Particularly good fluorescent dyes in commercial practice are coumarins such as derivatives of 7-dialkylaminocumarin, xanthenes such as fluorescein and its halogenation products eosine, erythrosine, phloxine and Rose bengal and rhodamines and in particular methine dyes such as cyanines.
For the fluorescence-analytical examination of surfaces on which other fluorescent compounds are adsorbed, the dye chosen will differ significantly in its fluorescence spectrum from those of the other adsorbed compounds. In the case of optical brighteners as used in paper, for example, the dye chosen would fluoresce in the red or near infrared region of the spectrum.
The reactive groups, of which at least one is attached to the chromophore, usefully include radicals which react with the nucleophilic amino or imino group, for example Michael systems, such as vinylsulfonyl or the acid sulfuric esters which form these under basic conditions by elimination. Also useful are reactive groups known from peptide chemistry, such as acetyl azide, aryl halide, dichlorotriazine, isothiocyanate, sulfonyl chloride and sulfosuccinimidyl ester radicals. For cases where the substrates have adsorbed a plurality of nucleophilically reacting compounds capable of competing with the amino and imino groups, the dyes used would have reactive radicals which are selective with regard to amino and imino groups.
Particularly useful in these cases are succinimidyl esters combining high selectivity with good stability in aqueous solutions.
It is possible in this connection to use such fluorescent dyes as are commercially available as labeling agents for biomolecules such as proteins and antibodies directly for dyeing. Examples of common commercial labeling agents are cyanines containing one or more succinimidyl ester radicals.
Succinimidyl esters are incidentally also preparable simply by reacting fluorescent dyes bearing carboxyl groups with N-hydroxysulfosuccinimides.
The surface is examined by applying a dye solution to a sample of the substrate to be examined. In those areas of the surface where compounds bearing amino or imino groups are adsorbed, the dye becomes attached. A subsequent rinsing step washes away unattached dye. This enables fluorescence microscopy to be used to obtain an image of the coverage of the surface.
Compounds having amino and/or imino groups that are advantageously determinable by dyeing with fluorescent dyes are oligo- and polyamines bearing amino or imino groups. The amino or imino groups are attached to aliphatic or aromatic chains and may contain further functional radicals or else quaternary ammonium ions as well as the amino groups. Such compounds are used as assistants in various applications.
Examples are polyvinylamines as described for example in U.S. Pat. No. 4,217,214, EP-A-071 050 and EP-A-0 216 387. These compounds are polymers of N-vinylcarboxamides or their copolymers with ethylenically unsaturated monomers that are converted into polyvinylamines after the polymerization by elimination of the carboxylic acid radicals. Such polyvinylamines are used for example as paper assistants for fixating water-soluble and -insoluble contraries and have average molecular weights M
w
of from 200 to 10 million.
Similarly readily dyeable oligo- and polyamines are polyethyleneimines as described in U.S. Pas. No. 2,182,306 or U.S. Pat. No. 3,203,910, which have average molecular weights M
w
of from 250 to 2 million, usually from 500 to 10,000, when used as assistants in various applications. An example is the use of polyethyleneimines as retention aids in papermaking.
It will be appreciated that derivatives of polyvinylamines and polyethyleneimines are similarly readily dyeable, as long as not all free amino groups have been derivatized. Derivatizing reactions include for example carboxymethylations and also reactions with alkyl epoxides as described in WO-A-97/40087 and WO-A-97/42229.
Readily detectable amines further include oligoamines obtained by condensation reaction of, for example, primary mono- or diamines or secondary diamines with epichlorohydrin. Further examples include the addition products of these amines with acrylonitrile and subsequent hydrogenation. Examples of the first type of oligoamine mentioned are partially quaternized piperazine condensates with epichlorohydrin that have an average molecular weight of from 200 to 70,000. These amines are used as textile assistants in the dyeing of cotton fibers.
The use of fluorescent dyes permits the detection of compounds bearing amino and/or imino groups that are adsorbed on any surfaces of substrates.
Examples are paper, wood, leather, fabrics composed of manufactured or natural fibers, hairs, fur, sheet-silicate, hard surfaces such as glass, metal, ceramic or else catalyst surfaces.
The surfaces may have become coated with the oligo- or polyamines for example by treatment with decontaminating or cleaning formulations. However, they may also have become coated in the course of a finish operation on fibers and fabrics or directly during, for example, papermaking.
Such oligo- and polyamines have different molecular weights, depending on application requirements. However, this is immaterial to the dyeing of the oligo- and polyamines on the substrate, since they are adsorbed on the surface. The limit of detection is not reached until the amines to be assayed are so readily detached
Mahr Norbert
Nörenberg Ralf
Schrof Wolfgang
Basf Aktiengesellschaft
Cole Monique T.
Oblon & Spivak, McClelland, Maier & Neustadt P.C.
Warden Jill
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