Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Reexamination Certificate
2005-11-09
2009-08-04
Davis, Ruth A (Department: 1651)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
C435S040500, C435S325000, C435S358000
Reexamination Certificate
active
07569339
ABSTRACT:
Production of biopharmaceuticals from CHO cells requires generation of master-, working- and post-production cell banks of high quality, partly under GMP conditions. An optimal cryopreservation strategy is needed for each new production cell line, particularly with regard to the desire to establish production processes that are completely devoid of serum or even any animal components and to ensure robust thaw performance for reliable production. Here we describe a novel strategy employing flow cytometric (FC) analysis of Annexin V-stained cells for high-throughput characterization of CHO cell banks. Our data show that this method enables evaluation of a cryopreservation procedure just 6 h after thaw.
REFERENCES:
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Fieder Juergen
Kaufmann Hitto
Otto Ralf
Boehringer Ingelheim Pharma GmbH & Co. KG
Davis Ruth A
Devlin Mary-Ellen M.
Kershner David L.
Morris Michael P.
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