Use of exonuclease and/or glycosylase as supplements to anti-pol

Details Use of exonuclease and/or glycosylase as supplements to anti-pol Use of exonuclease and/or glycosylase as supplements to anti-pol

1996-05-08

1999-11-16

Fredman, Jeffrey

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 536 243, 536 2431, 536 2432, 536 2433, C12P 1934, C12Q 168, C07H 2102

Patent

active

059856190

ABSTRACT:
The present invention provides admixtures and methods for PCR amplification of a target nucleic acid in which amplification efficiency is increased by including an antibody specific for a polymerization agent and at least one of an exonuclease and a glycosylase in the PCR reaction mix. Kits for amplification of a target nucleic acid are also provided.

REFERENCES:
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patent: 5418149 (1995-05-01), Gelfand
patent: 5605796 (1997-02-01), Chen et al.
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Stratagene catalog, p. 196, 1995.
Promega catalog, p. 55, 1993.

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