Use of exo-sample nucleotides in gene cloning

Details Use of exo-sample nucleotides in gene cloning Use of exo-sample nucleotides in gene cloning

1991-06-14

1992-08-11

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 4351723, C12P 1934, C12Q 168, C12N 1500, C12N 1511

Patent

active

051378140

ABSTRACT:
The present invention provides improved methods for manipulating recombinant DNA in gene cloning and expression. More specifically, the invention provides methods capable of altering a nucleic acid sequence present at the termini of a target sequence.

REFERENCES:
patent: 4873192 (1989-10-01), Kunkel
patent: 5035996 (1991-07-01), Hartley
Suzuki et al. (1986), an Introduction to Genetic Analysis, Third Edition (W. H. Freeman & Co., New York), p. 309.
Duncan, B., The Enzymes XIV:565-586 (1981).
Longo, M. C. et al., Gene 93:125-128 (1990).
Frohman, M. A. et al., Proc. Natl. Acad. Sci. (U.S.A.) 85:8998-9002 (1988).
Ohara, O. et al., Proc. Natl. Acad. Sci. (U.S.A.) 86:5673-5677 (1989).

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