Use of estramustine phosphate in the treatment of bone...

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Phosphorus containing other than solely as part of an...

Reexamination Certificate

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C514S103000, C514S108000, C514S171000, C424S450000

Reexamination Certificate

active

06436913

ABSTRACT:

The present invention relates to the use of estramustine phosphate in the treatment of bone metastasis, particularly in the treatment of bone metastasis in patients with prostate cancer.
Although the success rate for curing primary cancers is increasing, metastasis remains a limiting factor in antitumour therapy. Metastasis involves the spread of cancer cells from the primary cancer site to a secondary location elsewhere in the body. A common secondary site for metastasising tumour cells is in the bone. The presence of malignant cells in bone induces metabolic bone disease leading, for example, to bone resorption. The clinical symptoms of bone metastasis such as bone pain are partly linked to bone resorption. It has therefore been found that bisphosphonates, which are specific inhibitors of osteoclast-mediated bone resorption, can relieve bone pain in patients with skeletal metastases from prostate cancer. Estramustine phosphate (The Merck Index, XII Ed., No. 3749, 1996) is an estradiol-17&bgr;-phosphate derivative widely known in the art as an antitumor agent, currently used in the treatment of advanced adenocarcinoma of the prostate.
As an example, initial intravenous administration of estramustine phosphate, followed by oral administration, has been reported at dosages paralleling the oral administration for the drug, i.e. 300-600 mg daily given intravenously and usually repetitively over for several consecutive days, or as a once weekly high dose of 1000-2500 mg/m
2
(see, for a reference, British Journal of Urology, 1977, 49, 73-79; J. Urol. 108:303-306, 1972; Eur. Clin. Pharmacol. 26(1), 113-119, 1984; Eur. Urol. 1990, 17, 216-218).
It has now been found that the intravenous estramustine phosphate can inhibit bone resorption and is thus useful in treating the symptoms of bone metastasis. Accordingly, the present invention provides the use of estramustine phosphate in the manufacture of a medicament for intravenous use as an inhibitor of bone resorption, for instance osteoclast-mediated bone resorption. The invention also provides a method of inhibiting bone resorption in a patient in need of such treatment, which method comprises the intravenous administration to the said patient of an effective amount of estramustine phosphate. The condition of the patient may thereby be improved. The invention also provides an agent for inhibiting bone resorption comprising intravenous estramustine phosphate.
In a particular embodiment of the present invention the medicament containing estramustine phosphate is used to treat, prevent or alleviate the symptoms of bone metastasis. The bone metastasis results from cancer elsewhere in the body, for example prostate cancer, breast cancer, melanoma, lung cancer, pancreatic cancer, colorectal cancer, ovarian cancer and cancers of the brain. In particular, the medicament is for treating, preventing or alleviating the symptoms of bone metastasis in a prostate cancer patient. More in particular, the medicament prevents or alleviates symptoms of pain associated with bone metastases and risk of pathological fractures. In the present invention, estramustine phosphate may be administered in the form of a pharmaceutically acceptable salt, for instance as sodium salt or as a salt with a basic amino acid, e.g. arginine, or with N-methyl glucamine, otherwise referred to as meglumine.
The dosage regimen for the compounds and/or compositions containing the compounds is based on a variety of factors, including the type, age, weight, sex and medical condition of the patient; the severity of the condition; the route of administration; and the activity of the particular compound employed. Thus the dosage regime may vary widely.
According to an embodiment of the invention, the estramustine phosphate formulation can be administered to patients either as a slow injection, e.g. over about 30 minutes to about 3 hours, or as a bolus injection, also referred to as IV (intravenous) push. The intravenous formulations of the present invention are prepared according to conventional techniques adopted in the preparation of pharmaceutical forms for parenteral use. Typically, a proper amount of estramustine phosphate, either as a dry powder or in a lyophilised form, is dissolved in a pharmaceutically acceptable solution for parenteral use.
As an example, a proper amount of estramustine phosphate in the form of a suitable salt such as, for instance, N-methyl glucamine salt, is dissolved in a suitable amount of sterile water or aqueous dextrose solution, e.g. 5% dextrose in water for intravenous administration.
Likewise, a proper amount of estramustine phosphate is dispersed in water and then dissolved by adding at least an equimolar amount of a basic amino acid, for instance arginine. A further amount of the given amino acid, e.g. arginine, can be present in order to reach an estramustine phosphate:arginine molar ratio higher than 1:1, respectively.
Alternatively, a proper amount of estramustine phosphate in the form of a pharmaceutically acceptable salt for parenteral use, e.g. estramustine phosphate meglumine salt, either as a dry powder or into a lyophilised form, is dissolved in a pharmaceutically acceptable solution for parenteral use, for instance sterile water or aqueous dextrose solution, e.g. 5% dextrose in water for intravenous administration, and then admixed with a proper amount of a basic amino acid, for instance arginine. The above mixture is then stirred, sterilised, and subsequently lyophilised according to conventional techniques. The freeze-dried formulation is prepared and stored in vials for injection; the addition of a proper amount of sterile water or a physiological solution for parenteral use enables the preparation of the final formulation to be injected.
The above method is also suitable for preparing high dosage estramustine phosphate formulations. The unit-strength of the formulation to be injected depends on the concentration of the active in the solution itself and, of course, on the filling volume of the vials used to prepare the final formulation.
The formulations comprising estramustine phosphate may optionally contain additional pharmaceutically acceptable excipients for parenteral administration such as, for instance, bulking agents, e.g. lactose or mannitol, pH buffering agents, anti-oxidant agents, preservative agents, tonicity adjusters and the like.
The formulations of the present invention allow the administration of the active principle either as a single agent or, alternatively, according to a combined chemotherapy regimen. As an example, the formulations can be for administration in combination with an additional chemotherapeutic agent selected from taxane, taxane derivatives, CPT-11, camptothecin derivatives, anthracycline glycosides, e.g. doxorubicin or epirubicin, etoposide, navelbine, vinblastine, carboplatin, cisplatin and the like, optionally within liposomal formulations thereof In one embodiment, the medicament of the present invention further comprises the said additional chemotherapeutic agent.
In addition to the above, the intravenous estramustine formulations of the invention may also be administered in combination with a bone resorption inhibitor, for instance with the aforementioned bisphosphonates such as clodronate, palmidronate or etridronate.
The invention also provides a product comprising estramustine phosphate and one or more chemotherapeutic agents, optionally within liposomal formulations thereof, and/or a bisphosphonate selected from taxane, taxane derivatives, CPT-11, camptothecin derivatives, anthracycline glycosides, etoposide, navelbine, vinblastine, carboplatin, cisplatin, clodronate, palmidronate and etridronate, as a combined preparation for simultaneous, separate or sequential administration in the inhibition of bone resorption. Such a combined preparation may, for instance, be used for treating, preventing or alleviating the symptoms of bone metastasis.
In accordance with the present invention, the medicament comprising estramustine phosphate may be given once weekly to a maximal dose of 4000 mg or

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