Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2011-08-30
2011-08-30
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200
Reexamination Certificate
active
08008019
ABSTRACT:
The methods and compositions of the present invention allow for the evaluation of a nucleotide expansion, contraction or deletion. In one embodiment, the present invention provides a method for detecting a nucleotide expansion, contraction or deletion comprising amplifying a target nucleic acid sequence with a primer pair, each primer comprising a target specific sequence and a differentiating tag sequence, labeling the target nucleic acid sequence to produce a dual-tagged amplification product; digesting the dual-tagged amplification product to produce an expansion region fragment and an internal control fragment; hybridizing the fragments to separate capture complexes; detecting the signals produced by the labels on the immobilized fragments and comparing the intensity of the signals to detect the nucleotide expansion, contraction or deletion region.
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Bortolin Susan
Galvan-Goldman Barbara
Merante Frank
Fulbright & Jaworski LLP
Horlick Kenneth R.
Luminex Molecular Diagnostics
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